Acute effect of electroacupuncture at the Zusanli acupoints on decreasing insulin resistance as shown by lowering plasma free fatty acid levels in steroid-background male rats
Language: 
English
Abstract: 

BACKGROUND: Insulin sensitivity has been enhanced by electroacupuncture (EA) in rats, but the EA phenomenon in an insulin resistant state is still unclear. This study reports the use of a large dose of prednisolone to evaluate the effects of EA in a state of insulin resistance. METHODS: The plasma levels of free fatty acids (FFAs) were estimated in steroid-background rats (SBRs) and compared with those in healthy rats treated with normal saline. In addition, plasma glucose and endogenous insulin levels were assayed to calculate the homeostasis model assessment (HOMA) index. Intravenous glucose tolerance test (IVGTT) was carried out to compare glucose tolerance. The SBRs were randomly divided into EA-treatment and non-EA treatment groups and 15-Hz EA was applied to the bilateral Zusanli acupoints to investigate its effects on insulin resistance. In addition to an insulin challenge test (ICT) and IVGTT, the plasma levels of FFAs were measured and western blot was performed to help determine the effects of EA on the insulin resistant state. RESULTS: The plasma levels of FFAs increased markedly in SBRs, the HOMA index was markedly higher, and glucose tolerance was impaired. EA improved glucose tolerance and insulin sensitivity by decreasing the plasma levels of FFAs. Further, the insulin signaling proteins (IRS1) and glucose transporter isoform protein (GLUT4) in skeletal muscle inhibited by prednisolone recovered after EA. CONCLUSION: Insulin resistance was successfully induced by a large dose of prednisolone in male rats. This insulin resistance can be improved by 15 Hz EA at the bilateral Zusanli acupoints, as shown by decreased plasma levels of FFAs.

Author(s): 
Lin, Rong-Tsung
Tzeng, Chung-Yuh
Lee, Yu-Chen
Ho, Wai-Jane
Cheng, Juei-Tang
Lin, Jaung-Geng
Chang, Shih-Liang
Item Type: 
Journal Article
Publication Title: 
BMC complementary and alternative medicine
Journal Abbreviation: 
BMC Complement Altern Med
Publication Date: 
2009
Publication Year: 
2009
Pages: 
26
Volume: 
9
ISSN: 
1472-6882
DOI: 
10.1186/1472-6882-9-26
Library Catalog: 
NCBI Published Medical (?)
Extra: 
PMID: 19646276 PMCID: PMC2731038

Turabian/Chicago Citation

Rong-Tsung Lin, Chung-Yuh Tzeng, Yu-Chen Lee, Wai-Jane Ho, Juei-Tang Cheng, Jaung-Geng Lin and Shih-Liang Chang. 2009. "Acute effect of electroacupuncture at the Zusanli acupoints on decreasing insulin resistance as shown by lowering plasma free fatty acid levels in steroid-background male rats." BMC complementary and alternative medicine 9: 26. 10.1186/1472-6882-9-26.

Wikipedia Citation

<ref> {{Cite journal | doi = 10.1186/1472-6882-9-26 | issn = 1472-6882 | volume = 9 | pages = 26 | last = Lin | first = Rong-Tsung | coauthors = Tzeng, Chung-Yuh, Lee, Yu-Chen, Ho, Wai-Jane, Cheng, Juei-Tang, Lin, Jaung-Geng, Chang, Shih-Liang | title = Acute effect of electroacupuncture at the Zusanli acupoints on decreasing insulin resistance as shown by lowering plasma free fatty acid levels in steroid-background male rats | journal = BMC complementary and alternative medicine | date = 2009 | pmid = | pmc = }} </ref>