Amplification of tandemly repeated origin control sequences confers a replication advantage on rDNA replicons in Tetrahymena thermophila
Language: 
English
Abstract: 

The macronuclear rRNA genes (rDNA) in the ciliate Tetrahymena thermophila are normally palindromic linear replicons, containing two copies of the replication origin region in inverted orientation. A circular plasmid containing a single Tetrahymena rRNA gene (one half palindrome) joined to a tandem repeat of a 1.9-kilobase (kb) rDNA segment encompassing the rDNA replication origin and known replication control elements was used to transform Tetrahymena macronuclei by microinjection. This plasmid was shown previously to have a replication advantage over the rDNA allele of the recipient cell strain (G.-L. Yu and E. H. Blackburn, Proc. Natl. Acad. Sci. USA 86:8487-8491, 1990). During vegetative cell divisions, the circular and palindromic rDNAs were rapidly replaced by novel, successively longer linear rDNAs that eventually contained up to 30 tandem 1.9-kb repeats, resulting from homologous but unequal crossovers between the 1.9-kb repeats. We present evidence to show that increasing the number of copies of the replication control regions increases the replicative advantage of the rDNA, the first such situation for a cellular nuclear replicon in a eucaryote.

Author(s): 
Yu, G. L.
Blackburn, E. H.
Item Type: 
Journal Article
Publication Title: 
Molecular and Cellular Biology
Journal Abbreviation: 
Mol. Cell. Biol.
Publication Date: 
1990-05
Publication Year: 
1990
Pages: 
2070-2080
Volume: 
10
Issue: 
5
ISSN: 
0270-7306
Library Catalog: 
NCBI Published Medical (?)
Extra: 
PMID: 2325646 PMCID: PMC360554

Turabian/Chicago Citation

G. L. Yu and E. H. Blackburn. 1990-05. "Amplification of tandemly repeated origin control sequences confers a replication advantage on rDNA replicons in Tetrahymena thermophila." Molecular and Cellular Biology 10: 5: 2070-2080.

Wikipedia Citation

<ref> {{Cite journal | doi = | issn = 0270-7306 | volume = 10 | pages = 2070-2080 | last = Yu | first = G. L. | coauthors = Blackburn, E. H. | title = Amplification of tandemly repeated origin control sequences confers a replication advantage on rDNA replicons in Tetrahymena thermophila | journal = Molecular and Cellular Biology | date = 1990-05 | pmid = | pmc = }} </ref>