Anti-hyperglycemic effect of chebulagic acid from the fruits of Terminalia chebula Retz
Language: 
English
Abstract: 

In the present study, we firstly compared rat intestinal ?-glucosidase inhibitory activity by different ethanol-aqueous extractions from the dried fruits of Terminalia chebula Retz. The enzymatic assay showed that the 80% ethanol extract was more potent against maltase activity than both 50% and 100% ethanol extracts. By HPLC analysis, it was determined that the 80% ethanol extract had a higher content of chebulagic acid than each of 50% or 100% ethanol extract. Next, we investigated how efficiently chebulagic acid could inhibit sugar digestion by determining the glucose level on the apical side of the Caco-2 cell monolayer. The result showed that the maltose-hydrolysis activity was down-regulated by chebulagic acid, which proved to be a reversible inhibitor of maltase in Caco-2 cells. On the other hand, chebulagic acid showed a weak inhibition of sucrose-hydrolysis activity. Meanwhile, chebulagic acid did not have an obvious influence on intestinal glucose uptake and was not effective on glucose transporters. Further animal studies revealed that the oral administration of chebulagic acid (100 mg/kg body weight) significantly reduced postprandial blood glucose levels by 11.1% in maltose-loaded Sprague-Dawley (SD) rats compared with the control group, whereas the oral administration of chebulagic acid did not show a suppressive effect on postprandial hyperglycemia in sucrose- or glucose-loaded SD-rats. The results presented here suggest that chebulagic acid from T. chebula can be used to control blood glucose and manage type 2 diabetes, although clinical trials are needed.

Author(s): 
Huang, Yi-Na
Zhao, Dong-Dong
Gao, Bo
Zhong, Kai
Zhu, Rui-Xue
Zhang, Yan
Xie, Wang-Jun
Jia, Li-Rong
Gao, Hong
Item Type: 
Journal Article
Publication Title: 
International Journal of Molecular Sciences
Journal Abbreviation: 
Int J Mol Sci
Publication Date: 
2012
Publication Year: 
2012
Pages: 
6320-6333
Volume: 
13
Issue: 
5
ISSN: 
1422-0067
DOI: 
10.3390/ijms13056320
Library Catalog: 
PubMed
Extra: 
PMID: 22754367 PMCID: PMC3382786

Turabian/Chicago Citation

Yi-Na Huang, Dong-Dong Zhao, Bo Gao, Kai Zhong, Rui-Xue Zhu, Yan Zhang, Wang-Jun Xie, Li-Rong Jia and Hong Gao. 2012. "Anti-hyperglycemic effect of chebulagic acid from the fruits of Terminalia chebula Retz." International Journal of Molecular Sciences 13: 5: 6320-6333. 10.3390/ijms13056320.

Wikipedia Citation

<ref> {{Cite journal | doi = 10.3390/ijms13056320 | issn = 1422-0067 | volume = 13 | pages = 6320-6333 | last = Huang | first = Yi-Na | coauthors = Zhao, Dong-Dong, Gao, Bo, Zhong, Kai, Zhu, Rui-Xue, Zhang, Yan, Xie, Wang-Jun, Jia, Li-Rong, Gao, Hong | title = Anti-hyperglycemic effect of chebulagic acid from the fruits of Terminalia chebula Retz | journal = International Journal of Molecular Sciences | date = 2012 | pmid = | pmc = }} </ref>