Antipruritic effect of cold stimulation at the Quchi Acupoint (LI11) in Mice

BACKGROUND: Acupuncture and moxibustion are used to treat pruritus and atopic dermatitis. However, whether cold stimulation (defined as that the temperature conducted under skin temperature) of acupoints affects itching in experimental murine models remains unclear. METHODS: The present study was designed to determine the therapeutic effects of different thermal stimulations at the Quchi acupoint (LI11) in a murine model in which scratching behaviour was elicited by subcutaneous injection with a pruritogenic agent (compound 48/80). Male ICR mice were divided into several groups as follows: control (saline), those receiving compound 48/80 and compound 48/80 with various thermal stimulations (5°C-45°C) at LI11 (n = 6 per group). The scratch response of each animal to these stimulations was recorded for 30 min. The antipruritic effect of the acupoint was further evaluated in LI11 and sham (non-acupoint) groups (n = 6 per group). RESULTS: Treatment with lower temperature (20°C) at the LI11 acupoint significantly attenuated compound 48/80-induced scratching; however, this antipruritic effect was not observed with stimulation at the sham point. The expression of c-fos in the neuron of the cervical spine induced by compound 48/80 was suppressed by cold stimulation at LI11. The antipruritic effect of cold stimulation was blocked by ruthium red (RR), a non-selective transient receptor potential (TRP) channel blocker, suggesting that TRP channels may play an important role in the antipruritic effect of cold stimulation at LI11 in mice. CONCLUSIONS: This study demonstrated that cold stimulation at LI11 attenuated compound 48/80-induced scratching behaviour in mice, possibly by a TRP-related pathway.

Tsai, Kao-Sung
Chen, Yung-Hsiang
Chen, Huey-Yi
Shen, Ein-Yiao
Lee, Yu-Chen
Shen, Jui-Lung
Wu, San-Yuan
Lin, Jaung-Geng
Chen, Yi-Hung
Chen, Wen-Chi
Item Type: 
Journal Article
Publication Title: 
BMC complementary and alternative medicine
Journal Abbreviation: 
BMC Complement Altern Med
Publication Date: 
Publication Year: 
Library Catalog: 
NCBI Published Medical (?)
PMID: 25239797 PMCID: PMC4179855

Turabian/Chicago Citation

Kao-Sung Tsai, Yung-Hsiang Chen, Huey-Yi Chen, Ein-Yiao Shen, Yu-Chen Lee, Jui-Lung Shen, San-Yuan Wu, Jaung-Geng Lin, Yi-Hung Chen and Wen-Chi Chen. 2014. "Antipruritic effect of cold stimulation at the Quchi Acupoint (LI11) in Mice." BMC complementary and alternative medicine 14: 1: 341. 10.1186/1472-6882-14-341.

Wikipedia Citation

<ref> {{Cite journal | doi = 10.1186/1472-6882-14-341 | issn = 1472-6882 | volume = 14 | pages = 341 | last = Tsai | first = Kao-Sung | coauthors = Chen, Yung-Hsiang, Chen, Huey-Yi, Shen, Ein-Yiao, Lee, Yu-Chen, Shen, Jui-Lung, Wu, San-Yuan, Lin, Jaung-Geng, Chen, Yi-Hung, Chen, Wen-Chi | title = Antipruritic effect of cold stimulation at the Quchi Acupoint (LI11) in Mice | journal = BMC complementary and alternative medicine | date = 2014 | pmid = | pmc = }} </ref>