[Apoptosis of glioma cell line U251 induced by small interfering RNA targeting survivin]
Language: 
Chinese
Abstract: 

OBJECTIVE: To construct recombinant expression vectors of small interfering RNA (siRNA) targeting survivin and investigate apoptosis of glioma cell line U251 mediated by the survivin-targeting siRNA. METHODS: According to the sequence of the coding region of survivin gene, two strings of 19 nucleotides of inverted sequence flanking the loop sequence of two complementary 9-base oligonucleotides were designed and synthesized to form hairpin construct as the DNA templates for the target siRNA. The siRNA templates were cloned into siRNA expression vector pGenesil-1, and the resulted vector pGenesil-1/survivin was transfected into U251 cells using Metafectene following the standard protocols. Real-time PCR and Western blotting were performed to evaluate survivin gene silencing induced by siRNA transfection at the RNA and protein levels, respectively. Flow cytometry analysis with Annexin-V/PI double staining was used to determine the cell apoptosis. RESULTS: Real-time RT-PCR and Western blotting revealed significantly lowered survivin expression at both RNA and protein levels in transfected U251 cells, which exhibited a significantly higher apoptosis rate after transfection as shown by flow cytometry analysis. CONCLUSION: RNA interference mediated by the siRNA expression vector pGenesi-l/survivin can significantly reduce survivin expression and induce remarkable apoptosis in U251 cells.

Author(s): 
Xu, Ru-xiang
Tu, Yan-yang
Jiang, Xiao-dan
Feng, Jiang-nan
Huang, Jun
Item Type: 
Journal Article
Publication Title: 
Nan Fang Yi Ke Da Xue Xue Bao = Journal of Southern Medical University
Journal Abbreviation: 
Nan Fang Yi Ke Da Xue Xue Bao
Publication Date: 
4/6/2015
Publication Year: 
2006
Pages: 
398-401
Volume: 
26
Issue: 
4
ISSN: 
1673-4254
Library Catalog: 
PubMed
Extra: 
PMID: 16624736

Turabian/Chicago Citation

Ru-xiang Xu, Yan-yang Tu, Xiao-dan Jiang, Jiang-nan Feng and Jun Huang. 4/6/2015. "[Apoptosis of glioma cell line U251 induced by small interfering RNA targeting survivin]." Nan Fang Yi Ke Da Xue Xue Bao = Journal of Southern Medical University 26: 4: 398-401.

Wikipedia Citation

<ref> {{Cite journal | doi = | issn = 1673-4254 | volume = 26 | pages = 398-401 | last = Xu | first = Ru-xiang | coauthors = Tu, Yan-yang, Jiang, Xiao-dan, Feng, Jiang-nan, Huang, Jun | title = [Apoptosis of glioma cell line U251 induced by small interfering RNA targeting survivin] | journal = Nan Fang Yi Ke Da Xue Xue Bao = Journal of Southern Medical University | date = 4/6/2015 | pmid = | pmc = }} </ref>