The aqueous extract of a popular herbal nutrient supplement, Angelica sinensis, protects mice against lethal endotoxemia and sepsis
Language: 
English
Abstract: 

Despite recent advances in antibiotic therapy and intensive care, sepsis remains a widespread problem in critically ill patients. The high mortality from sepsis is in part mediated by bacterial endotoxin, which stimulates macrophages/monocytes to sequentially release early (e.g., tumor necrosis factor, interleukin-1, and interferon-gamma) and late [e.g., high mobility group box 1 protein (HMGB1)] proinflammatory cytokines. Our discovery of HMGB1 as a late mediator of lethal systemic inflammation has initiated a new field of investigation for the development of experimental therapeutics. A popular Chinese herb, Angelica sinensis (also known as Dang Gui or Dong Quai) has been used traditionally for treating women with gynecological disorders (such as dysmenorrheal and hot flashes). Here we examined the effect of Angelica sinensis extract on endotoxin-induced HMGB1 release in vitro, and explored its therapeutic potential in animal models of lethal endotoxemia and sepsis [induced by cecal ligation and puncture (CLP)] in vivo. We demonstrated that a low-molecular-weight (<10 kDa) fraction of A. sinensis extract significantly attenuated endotoxin-induced HMGB1 release in part through interfering with its cytoplasmic translocation in macrophage cultures. Prophylactic administration of an aqueous extract of A. sinensis significantly attenuated systemic HMGB1 accumulation in vivo, and conferred a dose-dependent protection against lethal endotoxemia. Furthermore, delayed administration of A. sinensis extract beginning 24 h after CLP attenuated systemic HMGB1 accumulation, and significantly rescued mice from lethal sepsis. Taken together, these data suggest that A. sinensis contains water-soluble components that exert protective effects against lethal endotoxemia and experimental sepsis in part by attenuating systemic accumulation of a late proinflammatory cytokine, HMGB1.

Author(s): 
Wang, Haichao
Li, Wei
Li, Jianhua
Rendon-Mitchell, Beatriz
Ochani, Mahendar
Ashok, Mala
Yang, Lihong
Yang, Huan
Tracey, Kevin J.
Wang, Ping
Sama, Andrew E.
Item Type: 
Journal Article
Publication Title: 
The Journal of Nutrition
Journal Abbreviation: 
J. Nutr.
Publication Date: 
Feb 2006
Publication Year: 
2006
Pages: 
360-365
Volume: 
136
Issue: 
2
ISSN: 
0022-3166
Library Catalog: 
NCBI Published Medical (?)
Extra: 
PMID: 16424112 PMCID: PMC1766946

Turabian/Chicago Citation

Haichao Wang, Wei Li, Jianhua Li, Beatriz Rendon-Mitchell, Mahendar Ochani, Mala Ashok, Lihong Yang, Huan Yang, Kevin J. Tracey, Ping Wang and Andrew E. Sama. Feb 2006. "The aqueous extract of a popular herbal nutrient supplement, Angelica sinensis, protects mice against lethal endotoxemia and sepsis." The Journal of Nutrition 136: 2: 360-365.

Wikipedia Citation

<ref> {{Cite journal | doi = | issn = 0022-3166 | volume = 136 | pages = 360-365 | last = Wang | first = Haichao | coauthors = Li, Wei, Li, Jianhua, Rendon-Mitchell, Beatriz, Ochani, Mahendar, Ashok, Mala, Yang, Lihong, Yang, Huan, Tracey, Kevin J., Wang, Ping, Sama, Andrew E. | title = The aqueous extract of a popular herbal nutrient supplement, Angelica sinensis, protects mice against lethal endotoxemia and sepsis | journal = The Journal of Nutrition | date = Feb 2006 | pmid = | pmc = }} </ref>