Bifidobacteria isolated from infants and cultured on human milk oligosaccharides affect intestinal epithelial function
Language: 
English
Abstract: 

OBJECTIVES: Human milk oligosaccharides (HMOs) are the third most abundant component of breast milk. Our laboratory has previously revealed gene clusters specifically linked to HMO metabolism in selected bifidobacteria isolated from fecal samples of infants. Our objective was to test the hypothesis that growth of selected bifidobacteria on HMO stimulates the intestinal epithelium. METHODS: Caco-2 and HT-29 cells were incubated with lactose (LAC)- or HMO-grown Bifidobacterium longum subsp infantis (B infantis) or B bifidum. Bacterial adhesion and translocation were measured by real-time quantitative polymerase chain reaction. Expression of pro- and anti-inflammatory cytokines and tight junction proteins was analyzed by real-time reverse transcriptase. Distribution of tight junction proteins was measured using immunofluorescent microscopy. RESULTS: We showed that HMO-grown B infantis had a significantly higher rate of adhesion to HT-29 cells compared with B bifidum. B infantis also induced expression of a cell membrane glycoprotein, P-selectin glycoprotein ligand-1. Both B infantis and B bifidum grown on HMO caused less occludin relocalization and higher expression of anti-inflammatory cytokine, interleukin-10 compared with LAC-grown bacteria in Caco-2 cells. B bifidum grown on HMO showed higher expression of junctional adhesion molecule and occludin in Caco-2 cells and HT-29 cells. There were no significant differences between LAC or HMO treatments in bacterial translocation. CONCLUSIONS: The study provides evidence for the specific relation between HMO-grown bifidobacteria and intestinal epithelial cells. To our knowledge, this is the first study describing HMO-induced changes in the bifidobacteria-intestinal cells interaction.

Author(s): 
Chichlowski, Maciej
De Lartigue, Guillaume
German, J. Bruce
Raybould, Helen E.
Mills, David A.
Item Type: 
Journal Article
Publication Title: 
Journal of Pediatric Gastroenterology and Nutrition
Journal Abbreviation: 
J. Pediatr. Gastroenterol. Nutr.
Publication Date: 
Sep 2012
Publication Year: 
2012
Pages: 
321-327
Volume: 
55
Issue: 
3
ISSN: 
1536-4801
DOI: 
10.1097/MPG.0b013e31824fb899
Library Catalog: 
NCBI Published Medical (?)
Extra: 
PMID: 22383026 PMCID: PMC3381975

Turabian/Chicago Citation

Maciej Chichlowski, Guillaume De Lartigue, J. Bruce German, Helen E. Raybould and David A. Mills. Sep 2012. "Bifidobacteria isolated from infants and cultured on human milk oligosaccharides affect intestinal epithelial function." Journal of Pediatric Gastroenterology and Nutrition 55: 3: 321-327. 10.1097/MPG.0b013e31824fb899.

Wikipedia Citation

<ref> {{Cite journal | doi = 10.1097/MPG.0b013e31824fb899 | issn = 1536-4801 | volume = 55 | pages = 321-327 | last = Chichlowski | first = Maciej | coauthors = De Lartigue, Guillaume, German, J. Bruce, Raybould, Helen E., Mills, David A. | title = Bifidobacteria isolated from infants and cultured on human milk oligosaccharides affect intestinal epithelial function | journal = Journal of Pediatric Gastroenterology and Nutrition | date = Sep 2012 | pmid = | pmc = }} </ref>