Caloric restriction restores the chronological life span of the Goa1 null mutant of Candida albicans in spite of high cell levels of ROS
Language: 
English
Abstract: 

The Candida albicans Goa1p is required for mitochondrial functions. In a strain lacking GOA1 (GOA31), respiration, mitochondrial membrane potential, complex I (CI) activity of the electron transport chain, and ATP synthesis are significantly decreased. A shortened chronological life span (CLS) of GOA31 occurs in 2% glucose that is associated with an increase in cell reactive oxidant species (ROS) and apoptosis. We now show that caloric restriction (CR) in media containing 0.5% glucose instead of 2% glucose-SC extends the CLS to the level of parental and gene-reconstituted strains. Paradoxically, ROS levels in GOA31 far exceed those of control strains in 0.5% glucose and, as a consequence, increased lipid peroxidation occurs even though CLS is restored. Microarray analysis was used to characterize transcriptional changes during CR in GOA31. We found that CR shifts cells of all strains to a non-glucose carbon metabolism (?-oxidation). Our model of ROS formation in GOA31 follows the paradigm that the generation of oxygen radicals from ?-oxidation of cell lipids via FADH(2) (CII) and NADH (CI) creates an unfavorable cellular FADH(2)/NADH ratio that causes a transient overload in CII activity resulting in excess free cell radicals. In GOA31 the CI and peroxisomal dysfunctions increase the levels of ROS compared to control strains. Recovery from high levels of ROS may be associated with an increase in iron and sugar transporters, as well as an anti-stress response that includes the SOD1 and GPX1. Thus, CR creates a favorable growth environment, but cells of GOA31 must overcome a high but transient ROS production.

Author(s): 
Chen, Hui
Calderone, Richard
Sun, Nuo
Wang, Yun
Li, Dongmei
Item Type: 
Journal Article
Publication Title: 
Fungal genetics and biology: FG & B
Journal Abbreviation: 
Fungal Genet. Biol.
Publication Date: 
2012-12
Publication Year: 
2012
Pages: 
1023-1032
Volume: 
49
Issue: 
12
ISSN: 
1096-0937
DOI: 
10.1016/j.fgb.2012.09.007
Library Catalog: 
NCBI Published Medical (?)
Extra: 
PMID: 23063955

Turabian/Chicago Citation

Hui Chen, Richard Calderone, Nuo Sun, Yun Wang and Dongmei Li. 2012-12. "Caloric restriction restores the chronological life span of the Goa1 null mutant of Candida albicans in spite of high cell levels of ROS." Fungal genetics and biology: FG & B 49: 12: 1023-1032. 10.1016/j.fgb.2012.09.007.

Wikipedia Citation

<ref> {{Cite journal | doi = 10.1016/j.fgb.2012.09.007 | issn = 1096-0937 | volume = 49 | pages = 1023-1032 | last = Chen | first = Hui | coauthors = Calderone, Richard, Sun, Nuo, Wang, Yun, Li, Dongmei | title = Caloric restriction restores the chronological life span of the Goa1 null mutant of Candida albicans in spite of high cell levels of ROS | journal = Fungal genetics and biology: FG & B | date = 2012-12 | pmid = | pmc = }} </ref>