Chebulagic acid from Terminalia chebula enhances insulin mediated glucose uptake in 3T3-L1 adipocytes via PPAR? signaling pathway
Language: 
English
Abstract: 

The thiazolidinedione (TZDs) class of drugs are very effective for the treatment of type 2 diabetes mellitus (T2DM). But due to the adverse effects of synthetic TZDs, their use is strictly regulated. The therapeutic actions of TZDs are mediated via modulation of peroxisome proliferator-activated receptor gamma (PPAR?). Naturally occurring PPAR? modulators are more desirable as they lack the serious adverse effects caused by TZDs. This has prompted the exploitation of medicinal plants used in traditional medicine, for their potential PPAR? activity. In the present work, we studied chebulagic acid (CHA) isolated from fruits of Terminalia chebula with respect to its effect on adipogenesis, glucose transport, and endocrine function of adipocyte. The mRNA expression profile of PPAR? target gene CCAAT/enhancer-binding protein alpha (C/EBP-?) was analyzed by qRT-PCR. The putative binding mode and the potential ligand-target interactions of CHA, with PPAR? was analyzed using docking software (Autodock and iGEMDOCKv2). The results showed that CHA enhances PPAR? signaling and adipogenesis dose dependently but in a moderate way, less than rosiglitazone. GLUT4 expression and adiponectin secretion was increased by CHA treatment. The mRNA expression of PPAR? target gene C/EBP-? was increased in CHA -treated adipocytes. The comparison of results of various parameters of adipogenesis, insulin sensitivity, endocrine function and molecular docking experiments of roziglitazone and chebulagic acid indicate that the latter behaves like partial PPAR? agonist which could be exploited for phytoceutical development against T2DM.

Author(s): 
Shyni, Gangadharan Leela
Kavitha, Sasidharan
Indu, Sasidharan
Arya, Anil Das
Anusree, Sasidharan Suseela
Vineetha, Vadavanath Prabhakaran
Vandana, Sankar
Sundaresan, Andikannu
Raghu, Kozhiparambil Gopalan
Item Type: 
Journal Article
Publication Title: 
BioFactors (Oxford, England)
Journal Abbreviation: 
Biofactors
Publication Date: 
2014-12
Publication Year: 
2014
Pages: 
646-657
Volume: 
40
Issue: 
6
ISSN: 
1872-8081
DOI: 
10.1002/biof.1193
Library Catalog: 
PubMed
Extra: 
PMID: 25529897

Turabian/Chicago Citation

Gangadharan Leela Shyni, Sasidharan Kavitha, Sasidharan Indu, Anil Das Arya, Sasidharan Suseela Anusree, Vadavanath Prabhakaran Vineetha, Sankar Vandana, Andikannu Sundaresan and Kozhiparambil Gopalan Raghu. 2014-12. "Chebulagic acid from Terminalia chebula enhances insulin mediated glucose uptake in 3T3-L1 adipocytes via PPAR? signaling pathway." BioFactors (Oxford, England) 40: 6: 646-657. 10.1002/biof.1193.

Wikipedia Citation

<ref> {{Cite journal | doi = 10.1002/biof.1193 | issn = 1872-8081 | volume = 40 | pages = 646-657 | last = Shyni | first = Gangadharan Leela | coauthors = Kavitha, Sasidharan, Indu, Sasidharan, Arya, Anil Das, Anusree, Sasidharan Suseela, Vineetha, Vadavanath Prabhakaran, Vandana, Sankar, Sundaresan, Andikannu, Raghu, Kozhiparambil Gopalan | title = Chebulagic acid from Terminalia chebula enhances insulin mediated glucose uptake in 3T3-L1 adipocytes via PPAR? signaling pathway | journal = BioFactors (Oxford, England) | date = 2014-12 | pmid = | pmc = }} </ref>