Cloning and characterization of an autophagy-related gene, ATG12, from the three-host tick Haemaphysalis longicornis
Language: 
English
Abstract: 

Ticks are obligate hematophagous ectoparasites with a life cycle characterized by a period of starvation; many ticks spend more than 95% of their life off the host. Autophagy, which is the process of bulk cytoplasmic degradation in eukaryotic cells, is induced by starvation and is essential for extension of the lifespan. Therefore, we hypothesized that autophagy also occurs in ticks; however, there has been no report on autophagy-related (ATG) genes in ticks. Here, we show the homologue of an ATG gene, ATG12, and its expression pattern from the nymphal to adult stages in the three-host tick Haemaphysalis longicornis. The sequence analysis showed that H. longicornis ATG12 (HlATG12) cDNA is 649bp, has a 411bp ORF coding for a 136-amino acid polypeptide with the carboxy-terminal glycine residue, and has a predicted molecular mass of 15.2kDa. Moreover, RT-PCR revealed that HlATG12 was downregulated at the beginning of feeding, upregulated after engorgement, and downregulated again after molting. The expression level of HlATG12 was highest at 3 months after engorgement. By immuno-electron microscopy, it was demonstrated that HlAtg12 was localized to the region around granule-like structures within midgut cells of unfed adults. In conclusion, HlATG12 might function during unfed and molting stages.

Author(s): 
Umemiya, Rika
Matsuo, Tomohide
Hatta, Takeshi
Sakakibara, Shin-ichi
Boldbaatar, Damdinsuren
Fujisaki, Kozo
Item Type: 
Journal Article
Publication Title: 
Insect Biochemistry and Molecular Biology
Journal Abbreviation: 
Insect Biochem. Mol. Biol.
Publication Date: 
2007-09
Publication Year: 
2007
Pages: 
975-984
Volume: 
37
Issue: 
9
ISSN: 
0965-1748
DOI: 
10.1016/j.ibmb.2007.05.006
Library Catalog: 
NCBI Published Medical (?)
Extra: 
PMID: 17681237

Turabian/Chicago Citation

Rika Umemiya, Tomohide Matsuo, Takeshi Hatta, Shin-ichi Sakakibara, Damdinsuren Boldbaatar and Kozo Fujisaki. 2007-09. "Cloning and characterization of an autophagy-related gene, ATG12, from the three-host tick Haemaphysalis longicornis." Insect Biochemistry and Molecular Biology 37: 9: 975-984. 10.1016/j.ibmb.2007.05.006.

Wikipedia Citation

<ref> {{Cite journal | doi = 10.1016/j.ibmb.2007.05.006 | issn = 0965-1748 | volume = 37 | pages = 975-984 | last = Umemiya | first = Rika | coauthors = Matsuo, Tomohide, Hatta, Takeshi, Sakakibara, Shin-ichi, Boldbaatar, Damdinsuren, Fujisaki, Kozo | title = Cloning and characterization of an autophagy-related gene, ATG12, from the three-host tick Haemaphysalis longicornis | journal = Insect Biochemistry and Molecular Biology | date = 2007-09 | pmid = | pmc = }} </ref>