Colon-specific delivery of a probiotic-derived soluble protein ameliorates intestinal inflammation in mice through an EGFR-dependent mechanism
Language: 
English
Abstract: 

Probiotic bacteria can potentially have beneficial effects on the clinical course of several intestinal disorders, but our understanding of probiotic action is limited. We have identified a probiotic bacteria-derived soluble protein, p40, from Lactobacillus rhamnosus GG (LGG), which prevents cytokine-induced apoptosis in intestinal epithelial cells. In the current study, we analyzed the mechanisms by which p40 regulates cellular responses in intestinal epithelial cells and p40's effects on experimental colitis using mouse models. We show that the recombinant p40 protein activated EGFR, leading to Akt activation. Activation of EGFR by p40 was required for inhibition of cytokine-induced apoptosis in intestinal epithelial cells in vitro and ex vivo. Furthermore, we developed a pectin/zein hydrogel bead system to specifically deliver p40 to the mouse colon, which activated EGFR in colon epithelial cells. Administration of p40-containing beads reduced intestinal epithelial apoptosis and disruption of barrier function in the colon epithelium in an EGFR-dependent manner, thereby preventing and treating DSS-induced intestinal injury and acute colitis. Furthermore, p40 activation of EGFR was required for ameliorating colon epithelial cell apoptosis and chronic inflammation in oxazolone-induced colitis. These data define what we believe to be a previously unrecognized mechanism of probiotic-derived soluble proteins in protecting the intestine from injury and inflammation.

Author(s): 
Yan, Fang
Cao, Hanwei
Cover, Timothy L.
Washington, M. Kay
Shi, Yan
Liu, LinShu
Chaturvedi, Rupesh
Peek, Richard M.
Wilson, Keith T.
Polk, D. Brent
Item Type: 
Journal Article
Publication Title: 
The Journal of Clinical Investigation
Journal Abbreviation: 
J. Clin. Invest.
Publication Date: 
Jun 2011
Publication Year: 
2011
Pages: 
2242-2253
Volume: 
121
Issue: 
6
ISSN: 
1558-8238
DOI: 
10.1172/JCI44031
Library Catalog: 
NCBI Published Medical (?)
Extra: 
PMID: 21606592 PMCID: PMC3104743

Turabian/Chicago Citation

Fang Yan, Hanwei Cao, Timothy L. Cover, M. Kay Washington, Yan Shi, LinShu Liu, Rupesh Chaturvedi, Richard M. Peek, Keith T. Wilson and D. Brent Polk. Jun 2011. "Colon-specific delivery of a probiotic-derived soluble protein ameliorates intestinal inflammation in mice through an EGFR-dependent mechanism." The Journal of Clinical Investigation 121: 6: 2242-2253. 10.1172/JCI44031.

Wikipedia Citation

<ref> {{Cite journal | doi = 10.1172/JCI44031 | issn = 1558-8238 | volume = 121 | pages = 2242-2253 | last = Yan | first = Fang | coauthors = Cao, Hanwei, Cover, Timothy L., Washington, M. Kay, Shi, Yan, Liu, LinShu, Chaturvedi, Rupesh, Peek, Richard M., Wilson, Keith T., Polk, D. Brent | title = Colon-specific delivery of a probiotic-derived soluble protein ameliorates intestinal inflammation in mice through an EGFR-dependent mechanism | journal = The Journal of Clinical Investigation | date = Jun 2011 | pmid = | pmc = }} </ref>