Determination of artemether and dihydroartemisinin in human plasma with a new hydrogen peroxide stabilization method
Language: 
English
Abstract: 

BACKGROUND: Numerous methods have been reported for the determination of artemether (ARM) and its metabolite dihydroartemisinin (DHA) in plasma. However, stability issues in patient plasma have not received enough attention. RESULTS: An LC-MS/MS method for simultaneous determination of ARM and DHA in human plasma (K3EDTA) turned out to be problematic: ARM and DHA were degraded partially or completely in some patient plasma samples as indicated by the stable isotope-labeled internal standards. We postulated iron II (Fe(2+)) in hemoglobin or its derived products from malaria patients causes degradation of the drugs, and found that hydrogen peroxide (H2O2) protected the drugs from degradation. Acidifying plasma increased recovery of ARM significantly. Using only 50 µl of plasma sample, the method has a LLOQ at 0.5 ng/ml for both ARM and DHA. CONCLUSION: H2O2 is a stabilizing agent for artemisinin derivatives. The modified method is reliable and sensitive.

Author(s): 
Huang, Liusheng
Olson, Alexander
Gingrich, David
Aweeka, Francesca T.
Item Type: 
Journal Article
Publication Title: 
Bioanalysis
Journal Abbreviation: 
Bioanalysis
Publication Date: 
6/13/2015
Publication Year: 
2013
Pages: 
1501-1506
Volume: 
5
Issue: 
12
ISSN: 
1757-6199
DOI: 
10.4155/bio.13.91
Library Catalog: 
PubMed
Extra: 
PMID: 23795928 PMCID: PMC3879143

Turabian/Chicago Citation

Liusheng Huang, Alexander Olson, David Gingrich and Francesca T. Aweeka. 6/13/2015. "Determination of artemether and dihydroartemisinin in human plasma with a new hydrogen peroxide stabilization method." Bioanalysis 5: 12: 1501-1506. 10.4155/bio.13.91.

Wikipedia Citation

<ref> {{Cite journal | doi = 10.4155/bio.13.91 | issn = 1757-6199 | volume = 5 | pages = 1501-1506 | last = Huang | first = Liusheng | coauthors = Olson, Alexander, Gingrich, David, Aweeka, Francesca T. | title = Determination of artemether and dihydroartemisinin in human plasma with a new hydrogen peroxide stabilization method | journal = Bioanalysis | date = 6/13/2015 | pmid = | pmc = }} </ref>