Dihydroartemisinin (DHA) induces caspase-3-dependent apoptosis in human lung adenocarcinoma ASTC-a-1 cells
Language: 
English
Abstract: 

BACKGROUND: Dihydroartemisinin (DHA), a semi-synthetic derivative of artemisinin, isolated from the traditional Chinese herb Artemisia annua, is recommended as the first-line anti-malarial drug with low toxicity. DHA has been shown to possess promising anticancer activities and induce cancer cell death through apoptotic pathways, although the molecular mechanisms are not well understood. METHODS: In this study, cell counting kit (CCK-8) assay was employed to evaluate the survival of DHA-treated ASTC-a-1 cells. The induction of apoptosis was detected by Hoechst 33258 and PI staining as well as flow cytometry analysis. Collapse of mitochondrial transmembrane potential (DeltaPsim) was measured by dynamic detection under a laser scanning confocal microscope and flow cytometry analysis using Rhodamine123. Caspase-3 activities measured with or without Z-VAD-fmk (a broad spectrum caspase inhibitor) pretreatment by FRET techniques, caspase-3 activity measurement, and western blotting analysis. RESULTS: Our results indicated that DHA induced apoptotic cell death in a dose- and time-dependent manner, which was accompanied by mitochondrial morphology changes, the loss of DeltaPsim and the activation of caspase-3. CONCLUSION: These results show for the first time that DHA can inhibit proliferation and induce apoptosis via caspase-3-dependent mitochondrial death pathway in ASTC-a-1 cells. Our work may provide evidence for further studies of DHA as a possible anticancer drug in the clinical treatment of lung adenocarcinoma.

Author(s): 
Lu, Ying-Ying
Chen, Tong-Sheng
Qu, Jun-Le
Pan, Wen-Liang
Sun, Lei
Wei, Xun-Bin
Item Type: 
Journal Article
Publication Title: 
Journal of Biomedical Science
Journal Abbreviation: 
J. Biomed. Sci.
Publication Date: 
2009
Publication Year: 
2009
Pages: 
16
Volume: 
16
ISSN: 
1423-0127
DOI: 
10.1186/1423-0127-16-16
Library Catalog: 
PubMed
Extra: 
PMID: 19272183 PMCID: PMC2653522

Turabian/Chicago Citation

Ying-Ying Lu, Tong-Sheng Chen, Jun-Le Qu, Wen-Liang Pan, Lei Sun and Xun-Bin Wei. 2009. "Dihydroartemisinin (DHA) induces caspase-3-dependent apoptosis in human lung adenocarcinoma ASTC-a-1 cells." Journal of Biomedical Science 16: 16. 10.1186/1423-0127-16-16.

Wikipedia Citation

<ref> {{Cite journal | doi = 10.1186/1423-0127-16-16 | issn = 1423-0127 | volume = 16 | pages = 16 | last = Lu | first = Ying-Ying | coauthors = Chen, Tong-Sheng, Qu, Jun-Le, Pan, Wen-Liang, Sun, Lei, Wei, Xun-Bin | title = Dihydroartemisinin (DHA) induces caspase-3-dependent apoptosis in human lung adenocarcinoma ASTC-a-1 cells | journal = Journal of Biomedical Science | date = 2009 | pmid = | pmc = }} </ref>