Effect of ageing on pulmonary inflammation, airway hyperresponsiveness and T and B cell responses in antigen-sensitized and -challenged mice
Language: 
English
Abstract: 

BACKGROUND: The effect of ageing on several pathologic features of allergic asthma (pulmonary inflammation, eosinophilia, mucus hypersecretion), and their relationship with airway hyperresponsiveness (AHR) is not well characterized. OBJECTIVE: To evaluate lung inflammation, mucus metaplasia and AHR in relationship with age in murine models of allergic asthma comparing young and older mice. METHODS: Young (6 weeks) and older (6, 12, 18 months) BALB/c mice were sensitized and challenged with ovalbumin (OVA). AHR and bronchoalveolar fluid (BALF), total inflammatory cell count and differential were measured. To evaluate mucus metaplasia, quantitative PCR for the major airway mucin-associated gene, MUC-5AC, from lung tissue was measured, and lung tissue sections stained with periodic acid-Schiff (PAS) for goblet-cell enumeration. Lung tissue cytokine gene expression was determined by quantitative PCR, and systemic cytokine protein levels by ELISA from spleen-cell cultures. Antigen-specific serum IgE was determined by ELISA. RESULTS: AHR developed in both aged and young OVA-sensitized/challenged mice (OVA mice), and was more significantly increased in young OVA mice than in aged OVA mice. However, BALF eosinophil numbers were significantly higher, and lung histology showed greater inflammation in aged OVA mice than in young OVA mice. MUC-5AC expression and numbers of PAS+ staining bronchial epithelial cells were significantly increased in the aged OVA mice. All aged OVA mice had increased IL-5 and IFN-gamma mRNA expression in the lung and IL-5 and IFN-gamma protein levels from spleen cell cultures compared with young OVA mice. OVA-IgE was elevated to a greater extent in aged OVA mice. CONCLUSIONS: Although pulmonary inflammation and mucus metaplasia after antigen sensitization/challenge occurred to a greater degree in older mice, the increase in AHR was significantly less compared with younger OVA mice. Antigen treatment produced a unique cytokine profile in older mice (elevated IFN-gamma and IL-5) compared with young mice (elevated IL-4 and IL-13). Thus, the airway response to inflammation is lessened in ageing animals, and may represent age-associated events leading to different phenotypes in response to antigen provocation.

Author(s): 
Busse, Paula J.
Zhang, Teng Fei
Srivastava, Kamal
Schofield, Brian
Li, Xiu-Min
Item Type: 
Journal Article
Publication Title: 
Clinical and Experimental Allergy: Journal of the British Society for Allergy and Clinical Immunology
Journal Abbreviation: 
Clin. Exp. Allergy
Publication Date: 
Sep 2007
Publication Year: 
2007
Pages: 
1392-1403
Volume: 
37
Issue: 
9
ISSN: 
0954-7894
DOI: 
10.1111/j.1365-2222.2007.02775.x
Library Catalog: 
NCBI Published Medical (?)
Extra: 
PMID: 17845421 PMCID: PMC2818115

Turabian/Chicago Citation

Paula J. Busse, Teng Fei Zhang, Kamal Srivastava, Brian Schofield and Xiu-Min Li. Sep 2007. "Effect of ageing on pulmonary inflammation, airway hyperresponsiveness and T and B cell responses in antigen-sensitized and -challenged mice." Clinical and Experimental Allergy: Journal of the British Society for Allergy and Clinical Immunology 37: 9: 1392-1403. 10.1111/j.1365-2222.2007.02775.x.

Wikipedia Citation

<ref> {{Cite journal | doi = 10.1111/j.1365-2222.2007.02775.x | issn = 0954-7894 | volume = 37 | pages = 1392-1403 | last = Busse | first = Paula J. | coauthors = Zhang, Teng Fei, Srivastava, Kamal, Schofield, Brian, Li, Xiu-Min | title = Effect of ageing on pulmonary inflammation, airway hyperresponsiveness and T and B cell responses in antigen-sensitized and -challenged mice | journal = Clinical and Experimental Allergy: Journal of the British Society for Allergy and Clinical Immunology | date = Sep 2007 | pmid = | pmc = }} </ref>