Effect of interaction of magnetic nanoparticles of Fe?O? and artesunate on apoptosis of K562 cells
Language: 
English
Abstract: 

The present study evaluated whether the magnetic nanoparticles of Fe(3)O(4) (MNPs-Fe(3)O(4)) could enhance the activity of artesunate (ART), and to explore its potential mechanisms. Cytotoxicity of the copolymer of ART with MNPs-Fe(3)O(4) on K562 cells was detected by MTT assay and the apoptosis rate of K562 cells was measured by flow cytometry. Protein expression levels of bcl-2, bax, bcl-rambo, caspase-3, and survivin in K562 cells were measured by Western blot. After being incubated with the copolymer of ART with MNPs-Fe(3)O(4) for 48 hours, the growth inhibition rate of K562 cells was significantly increased compared with that of K562 cells treated with ART alone (P < 0.05), and the apoptosis rate of K562 cells was increased significantly compared with that of K562 cells treated with ART alone, suggesting that MNPs-Fe(3)O(4) can enhance the activity of ART. Interestingly, the copolymer-induced cell death was attenuated by caspase inhibitor Z-VAD-FMK. Our results also showed that treatment with the copolymer of MNPs-Fe(3)O(4) and ART increased the expression of bcl-2, bax, bcl-rambo, and caspase-3 proteins, and decreased the expression of survivin protein in K562 cells compared with ART treatment alone. These results suggest that MNPs-Fe(3)O(4) can enhance ART-induced apoptosis, which may be related to the upregulation of bcl-rambo and downregulation of survivin.

Author(s): 
Wang, Ying
Han, Yuxiang
Yang, Yingying
Yang, Jingci
Guo, Xiaonan
Zhang, Jingnan
Pan, Ling
Xia, Guohua
Chen, Baoan
Item Type: 
Journal Article
Publication Title: 
International Journal of Nanomedicine
Journal Abbreviation: 
Int J Nanomedicine
Publication Date: 
2011
Publication Year: 
2011
Pages: 
1185-1192
Volume: 
6
ISSN: 
1178-2013
DOI: 
10.2147/IJN.S19723
Library Catalog: 
PubMed
Extra: 
PMID: 21822380 PMCID: PMC3148844

Turabian/Chicago Citation

Ying Wang, Yuxiang Han, Yingying Yang, Jingci Yang, Xiaonan Guo, Jingnan Zhang, Ling Pan, Guohua Xia and Baoan Chen. 2011. "Effect of interaction of magnetic nanoparticles of Fe?O? and artesunate on apoptosis of K562 cells." International Journal of Nanomedicine 6: 1185-1192. 10.2147/IJN.S19723.

Wikipedia Citation

<ref> {{Cite journal | doi = 10.2147/IJN.S19723 | issn = 1178-2013 | volume = 6 | pages = 1185-1192 | last = Wang | first = Ying | coauthors = Han, Yuxiang, Yang, Yingying, Yang, Jingci, Guo, Xiaonan, Zhang, Jingnan, Pan, Ling, Xia, Guohua, Chen, Baoan | title = Effect of interaction of magnetic nanoparticles of Fe?O? and artesunate on apoptosis of K562 cells | journal = International Journal of Nanomedicine | date = 2011 | pmid = | pmc = }} </ref>