Effect of labeling with iron oxide particles or nanodiamonds on the functionality of adipose-derived mesenchymal stem cells
Language: 
English
Abstract: 

Stem cells are increasingly the focus of translational research as well as having emerging roles in human cellular therapy. To support these uses there is a need for improved methods for in vivo cell localization and tracking. In this study, we examined the effects of cell labeling on the in vitro functionality of human adipose-derived mesenchymal stem cells. Our results provide a basis for future in vivo studies investigating implanted cell fate and longevity. In particular, we investigated the effects of two different particles: micron-sized (~0.9 µm) fluorescently labeled (Dragon Green) superparamagnetic iron oxide particles (M-SPIO particles); and, carboxylated nanodiamonds of ~0.25 µm in size. The effects of labeling on the functionality of adipose-derived MSCs were assessed by in vitro morphology, osteogenic and adipogenic differentiation potential, CD marker expression, cytokine secretion profiling and quantitative proteomics of the intra-cellular proteome. The differentiation and CD marker assays for stem-like functionality were not altered upon label incorporation and no secreted or intra-cellular protein changes indicative of stress or toxicity were detected. These in vitro results indicate that the M-SPIO particles and nanodiamonds investigated in this study are biocompatible with MSCs and therefore would be suitable labels for cell localization and tracking in vivo.

Author(s): 
Blaber, Sinead P.
Hill, Cameron J.
Webster, Rebecca A.
Say, Jana M.
Brown, Louise J.
Wang, Shih-Chang
Vesey, Graham
Herbert, Benjamin Ross
Item Type: 
Journal Article
Publication Title: 
PloS One
Journal Abbreviation: 
PLoS ONE
Publication Date: 
2013
Publication Year: 
2013
Pages: 
e52997
Volume: 
8
Issue: 
1
ISSN: 
1932-6203
DOI: 
10.1371/journal.pone.0052997
Library Catalog: 
NCBI Published Medical (?)
Extra: 
PMID: 23301012 PMCID: PMC3536808

Turabian/Chicago Citation

Sinead P. Blaber, Cameron J. Hill, Rebecca A. Webster, Jana M. Say, Louise J. Brown, Shih-Chang Wang, Graham Vesey and Benjamin Ross Herbert. 2013. "Effect of labeling with iron oxide particles or nanodiamonds on the functionality of adipose-derived mesenchymal stem cells." PloS One 8: 1: e52997. 10.1371/journal.pone.0052997.

Wikipedia Citation

<ref> {{Cite journal | doi = 10.1371/journal.pone.0052997 | issn = 1932-6203 | volume = 8 | pages = e52997 | last = Blaber | first = Sinead P. | coauthors = Hill, Cameron J., Webster, Rebecca A., Say, Jana M., Brown, Louise J., Wang, Shih-Chang, Vesey, Graham, Herbert, Benjamin Ross | title = Effect of labeling with iron oxide particles or nanodiamonds on the functionality of adipose-derived mesenchymal stem cells | journal = PloS One | date = 2013 | pmid = | pmc = }} </ref>