Effects of 2-deoxyglucose and dehydroepiandrosterone on intracellular NAD(+) level, SIRT1 activity and replicative lifespan of human Hs68 cells
Language: 
English
Abstract: 

2-Deoxy-D-glucose (2-DG) and dehydroepiandrosterone (DHEA) have been hypothesized to extend lifespan via mimicking calorie restriction (CR). Activation of sirtuins has been proposed to contribute to life extension of CR by increasing intercellular levels of NAD(+) in several organisms. However, it is unclear whether 2-DG and DHEA may affect intracellular NAD(+) levels and human sirtuin 1 (SIRT1) activities. Here, using human fibroblast Hs68 cells we showed that 2-DG increased intracellular NAD(+) levels in both time- and concentration-dependent manners. 2-DG also dose-dependently increased SIRT1 activities and the lifespan (measured as the cumulated growth curve of population doubling levels) of Hs68 cells. In contrast, DHEA at non-cytotoxic concentrations (?50 ?M) did not significantly affect NAD(+) levels, SIRT1 activities or the lifespan of Hs68 cells. These results suggest that 2-DG extends the lifespan of Hs68 cells by increased NAD(+) levels and SIRT1 activities, and that 2-DG has a potential as a CR mimetic.

Author(s): 
Yang, Nae-Cherng
Song, Tuzz-Ying
Chen, Mei-Yau
Hu, Miao-Lin
Item Type: 
Journal Article
Publication Title: 
Biogerontology
Journal Abbreviation: 
Biogerontology
Publication Date: 
2011-12
Publication Year: 
2011
Pages: 
527-536
Volume: 
12
Issue: 
6
ISSN: 
1573-6768
DOI: 
10.1007/s10522-011-9342-7
Library Catalog: 
NCBI Published Medical (?)
Extra: 
PMID: 21604001

Turabian/Chicago Citation

Nae-Cherng Yang, Tuzz-Ying Song, Mei-Yau Chen and Miao-Lin Hu. 2011-12. "Effects of 2-deoxyglucose and dehydroepiandrosterone on intracellular NAD(+) level, SIRT1 activity and replicative lifespan of human Hs68 cells." Biogerontology 12: 6: 527-536. 10.1007/s10522-011-9342-7.

Wikipedia Citation

<ref> {{Cite journal | doi = 10.1007/s10522-011-9342-7 | issn = 1573-6768 | volume = 12 | pages = 527-536 | last = Yang | first = Nae-Cherng | coauthors = Song, Tuzz-Ying, Chen, Mei-Yau, Hu, Miao-Lin | title = Effects of 2-deoxyglucose and dehydroepiandrosterone on intracellular NAD(+) level, SIRT1 activity and replicative lifespan of human Hs68 cells | journal = Biogerontology | date = 2011-12 | pmid = | pmc = }} </ref>