Effects of chebulic acid on advanced glycation endproducts-induced collagen cross-links

Advanced glycation end-products (AGEs) have been implicated in the development of diabetic complications. We report the antiglycating activity of chebulic acid (CA), isolated from Terminalia chebula on breaking the cross-links of proteins induced by AGEs and inhibiting the formation of AGEs. Aminoguanidine (AG) reduced 50% of glycated bovine serum albumin (BSA) with glycolaldehyde (glycol-BSA)-induced cross-links of collagen at a concentration of 67.8 ± 2.5 mM, the level of CA required for exerting a similar antiglycating activity was 38.8 ± 0.5 µM. Also, the breaking activity on collagen cross-links induced by glycol-BSA was potent with CA (IC50=1.46 ± 0.05 mM), exhibiting 50-fold stronger breaking activity than with ALT-711, a well-known cross-link breaker (IC50=72.2 ± 2.4 mM). IC50 values of DPPH· scavenging activity for CA and ascorbic acid (AA) were 39.2 ± 4.9 and 19.0 ± 1.2 µg dry matter (DM) mL(-1), respectively, and ferric reducing and antioxidant power (FRAP) activities for CA and AA were 4.70 ± 0.06 and 11.4 ± 0.1 mmol/FeSO4·7H2O/g DM, respectively. The chelating activities of CA, AG and ALT711 on copper-catalyzed oxidation of AA were compared, and in increasing order, ALT-711 (IC50 of 1.92 ± 0.20 mM)<CA (IC50 of 0.96 ± 0.07 mM)<AG (0.47 ± 0.05 mM). Thus, CA could be a breaker as well as an inhibitor of AGE cross-linking, the activity of which may be explained in large part by its chelating and antioxidant activities, suggesting that CA may constitute a promising antiglycating candidate in intervening AGE-mediated diabetic complications.

Lee, Ji-Young
Oh, Jun-Gu
Kim, Jin Sook
Lee, Kwang-Won
Item Type: 
Journal Article
Publication Title: 
Biological & Pharmaceutical Bulletin
Journal Abbreviation: 
Biol. Pharm. Bull.
Publication Date: 
Publication Year: 
Library Catalog: 
PMID: 24759763

Turabian/Chicago Citation

Ji-Young Lee, Jun-Gu Oh, Jin Sook Kim and Kwang-Won Lee. 2014. "Effects of chebulic acid on advanced glycation endproducts-induced collagen cross-links." Biological & Pharmaceutical Bulletin 37: 7: 1162-1167.

Wikipedia Citation

<ref> {{Cite journal | doi = | issn = 1347-5215 | volume = 37 | pages = 1162-1167 | last = Lee | first = Ji-Young | coauthors = Oh, Jun-Gu, Kim, Jin Sook, Lee, Kwang-Won | title = Effects of chebulic acid on advanced glycation endproducts-induced collagen cross-links | journal = Biological & Pharmaceutical Bulletin | date = 2014 | pmid = | pmc = }} </ref>