An engineering approach to extending lifespan in C. elegans
Language: 
English
Abstract: 

We have taken an engineering approach to extending the lifespan of Caenorhabditis elegans. Aging stands out as a complex trait, because events that occur in old animals are not under strong natural selection. As a result, lifespan can be lengthened rationally using bioengineering to modulate gene expression or to add exogenous components. Here, we engineered longer lifespan by expressing genes from zebrafish encoding molecular functions not normally present in worms. Additionally, we extended lifespan by increasing the activity of four endogenous worm aging pathways. Next, we used a modular approach to extend lifespan by combining components. Finally, we used cell- and worm-based assays to analyze changes in cell physiology and as a rapid means to evaluate whether multi-component transgenic lines were likely to have extended longevity. Using engineering to add novel functions and to tune endogenous functions provides a new framework for lifespan extension that goes beyond the constraints of the worm genome.

Author(s): 
Sagi, Dror
Kim, Stuart K.
Item Type: 
Journal Article
Publication Title: 
PLoS genetics
Journal Abbreviation: 
PLoS Genet.
Publication Date: 
2012
Publication Year: 
2012
Pages: 
e1002780
Volume: 
8
Issue: 
6
ISSN: 
1553-7404
DOI: 
10.1371/journal.pgen.1002780
Library Catalog: 
NCBI Published Medical (?)
Extra: 
PMID: 22737090 PMCID: PMC3380832

Turabian/Chicago Citation

Dror Sagi and Stuart K. Kim. 2012. "An engineering approach to extending lifespan in C. elegans." PLoS genetics 8: 6: e1002780. 10.1371/journal.pgen.1002780.

Wikipedia Citation

<ref> {{Cite journal | doi = 10.1371/journal.pgen.1002780 | issn = 1553-7404 | volume = 8 | pages = e1002780 | last = Sagi | first = Dror | coauthors = Kim, Stuart K. | title = An engineering approach to extending lifespan in C. elegans | journal = PLoS genetics | date = 2012 | pmid = | pmc = }} </ref>