Epigenetic dysregulation of dopaminergic genes in eating disorders
Language: 
English
Abstract: 

OBJECTIVE: The pathophysiology of eating disorders such as anorexia nervosa (AN) and bulimia nervosa (BN) has been linked to an impaired dopaminergic neurotransmission, still the origin of this disturbance remains unknown. The aim of the present study was, therefore, to evaluate whether the expression of dopaminergic genes is altered in the blood of patients suffering from eating disorders and if these alterations can be explained by changes in the promoter specific DNA methylation of the genes. METHOD: We used quantitative real-time PCR to measure both the expression and the promoter specific DNA methylation of the dopamine transporter (DAT), and the D2 (DRD2) and D4 receptor (DRD4) gene in the blood of 46 patients (22 AN, 24 BN) and 30 healthy controls. RESULTS: Patients showed an elevated expression of DAT mRNA when compared with the controls and a downregulation of the DRD? expression. The upregulation of the DAT gene was accompanied by a hypermethylation of the gene's promoter in the AN and BN group while a significant hypermethylation of the DRD? promoter was only present in the AN group. No differences in expression or methylation were found for the other dopamine receptors investigated. DISCUSSION: Our study shows a disturbed expression of dopaminergic genes that is accompanied by a dysregulation of the epigenetic DNA methylation. Further studies are necessary to provide more insight into the epigenetic dysregulation of the dopaminergic neurotransmission in the pathophysiology of eating disorders.

Author(s): 
Frieling, Helge
Rˆmer, Konstanze D.
Scholz, Sarah
Mittelbach, Franziska
Wilhelm, Julia
De Zwaan, Martina
Jacoby, Georg E.
Kornhuber, Johannes
Hillemacher, Thomas
Bleich, Stefan
Item Type: 
Journal Article
Publication Title: 
The International Journal of Eating Disorders
Journal Abbreviation: 
Int J Eat Disord
Publication Date: 
11/1/2010
Publication Year: 
2010
Pages: 
577-583
Volume: 
43
Issue: 
7
ISSN: 
1098-108X
DOI: 
10.1002/eat.20745
Library Catalog: 
PubMed
Extra: 
PMID: 19728374

Turabian/Chicago Citation

Helge Frieling, Konstanze D. Rˆmer, Sarah Scholz, Franziska Mittelbach, Julia Wilhelm, Martina De Zwaan, Georg E. Jacoby, Johannes Kornhuber, Thomas Hillemacher and Stefan Bleich. 11/1/2010. "Epigenetic dysregulation of dopaminergic genes in eating disorders." The International Journal of Eating Disorders 43: 7: 577-583. 10.1002/eat.20745.

Wikipedia Citation

<ref> {{Cite journal | doi = 10.1002/eat.20745 | issn = 1098-108X | volume = 43 | pages = 577-583 | last = Frieling | first = Helge | coauthors = Rˆmer, Konstanze D., Scholz, Sarah, Mittelbach, Franziska, Wilhelm, Julia, De Zwaan, Martina, Jacoby, Georg E., Kornhuber, Johannes, Hillemacher, Thomas, Bleich, Stefan | title = Epigenetic dysregulation of dopaminergic genes in eating disorders | journal = The International Journal of Eating Disorders | date = 11/1/2010 | pmid = | pmc = }} </ref>