Eucommia ulmoides Oliver extract, aucubin, and geniposide enhance lysosomal activity to regulate ER stress and hepatic lipid accumulation
Language: 
English
Abstract: 

Eucommia ulmoides Oliver is a natural product widely used as a dietary supplement and medicinal plant. Here, we examined the potential regulatory effects of Eucommia ulmoides Oliver extracts (EUE) on hepatic dyslipidemia and its related mechanisms by in vitro and in vivo studies. EUE and its two active constituents, aucubin and geniposide, inhibited palmitate-induced endoplasmic reticulum (ER) stress, reducing hepatic lipid accumulation through secretion of apolipoprotein B and associated triglycerides and cholesterol in human HepG2 hepatocytes. To determine how EUE diminishes the ER stress response, lysosomal and proteasomal protein degradation activities were analyzed. Although proteasomal activity was not affected, lysosomal enzyme activities including V-ATPase were significantly increased by EUE as well as aucubin and geniposide in HepG2 cells. Treatment with the V-ATPase inhibitor, bafilomycin, reversed the inhibition of ER stress, secretion of apolipoprotein B, and hepatic lipid accumulation induced by EUE or its component, aucubin or geniposide. In addition, EUE was determined to regulate hepatic dyslipidemia by enhancing lysosomal activity and to regulate ER stress in rats fed a high-fat diet. Together, these results suggest that EUE and its active components enhance lysosomal activity, resulting in decreased ER stress and hepatic dyslipidemia.

Author(s): 
Lee, Hwa-Young
Lee, Geum-Hwa
Lee, Mi-Rin
Kim, Hye-Kyung
Kim, Nan-young
Kim, Seung-Hyun
Lee, Yong-Chul
Kim, Hyung-Ryong
Chae, Han-Jung
Item Type: 
Journal Article
Publication Title: 
PloS One
Journal Abbreviation: 
PLoS ONE
Publication Date: 
2013
Publication Year: 
2013
Pages: 
e81349
Volume: 
8
Issue: 
12
ISSN: 
1932-6203
DOI: 
10.1371/journal.pone.0081349
Library Catalog: 
NCBI Published Medical (?)
Extra: 
PMID: 24349058 PMCID: PMC3859480

Turabian/Chicago Citation

Hwa-Young Lee, Geum-Hwa Lee, Mi-Rin Lee, Hye-Kyung Kim, Nan-young Kim, Seung-Hyun Kim, Yong-Chul Lee, Hyung-Ryong Kim and Han-Jung Chae. 2013. "Eucommia ulmoides Oliver extract, aucubin, and geniposide enhance lysosomal activity to regulate ER stress and hepatic lipid accumulation." PloS One 8: 12: e81349. 10.1371/journal.pone.0081349.

Wikipedia Citation

<ref> {{Cite journal | doi = 10.1371/journal.pone.0081349 | issn = 1932-6203 | volume = 8 | pages = e81349 | last = Lee | first = Hwa-Young | coauthors = Lee, Geum-Hwa, Lee, Mi-Rin, Kim, Hye-Kyung, Kim, Nan-young, Kim, Seung-Hyun, Lee, Yong-Chul, Kim, Hyung-Ryong, Chae, Han-Jung | title = Eucommia ulmoides Oliver extract, aucubin, and geniposide enhance lysosomal activity to regulate ER stress and hepatic lipid accumulation | journal = PloS One | date = 2013 | pmid = | pmc = }} </ref>