Extension of the lifespan of cultured normal human diploid cells by vitamin E
Language: 
English
Abstract: 

Inclusion of vitamin E (DL-alpha-tocopherol) in the culture medium for human diploid cells greatly prolongs their in vitro lifespan. The addition of 100 mug of DL-alpha-tocopherol per ml of medium has allowed us to culture WI-38 cells for more than 100 population doublings to date. (These cells normally have an in vitro lifespan of 50 +/- 10 population doublings.) Cells at the 100th population doubling have a normal diploid karyotype, appear to behave in all other respects like young WI-38 cells, and are still actively dividing. We interpret this result as support for the free radical theory of aging.

Author(s): 
Packer, L.
Smith, J. R.
Item Type: 
Journal Article
Publication Title: 
Proceedings of the National Academy of Sciences of the United States of America
Journal Abbreviation: 
Proc. Natl. Acad. Sci. U.S.A.
Publication Date: 
1974-12
Publication Year: 
1974
Pages: 
4763-4767
Volume: 
71
Issue: 
12
ISSN: 
0027-8424
Library Catalog: 
NCBI Published Medical (?)
Extra: 
PMID: 4531015 PMCID: PMC433977

Turabian/Chicago Citation

L. Packer and J. R. Smith. 1974-12. "Extension of the lifespan of cultured normal human diploid cells by vitamin E." Proceedings of the National Academy of Sciences of the United States of America 71: 12: 4763-4767.

Wikipedia Citation

<ref> {{Cite journal | doi = | issn = 0027-8424 | volume = 71 | pages = 4763-4767 | last = Packer | first = L. | coauthors = Smith, J. R. | title = Extension of the lifespan of cultured normal human diploid cells by vitamin E | journal = Proceedings of the National Academy of Sciences of the United States of America | date = 1974-12 | pmid = | pmc = }} </ref>