Extension of the lifespan of cultured normal human diploid cells by vitamin E: a reevaluation
Language: 
English
Short Title: 
Extension of the lifespan of cultured normal human diploid cells by vitamin E
Abstract: 

Previously we reported [Packer, L. & Smith, J.R. (1974) Proc. Natl. Acad. Sci. USA 71, 4763-4767] that the lifespan of WI-38 human diploid fibroblasts in vitro was significantly increased by continuously growing the cell cultures in the presence of vitamin E(dl-alpha-tocopherol), but in 19 subsequent subcultivation series we were unable to reproduce these findings. While vitamin E is incorporated into the cells and is able to act effectively as an antioxidant, apparantly is intracellular antioxidant properties alone do not routinely result in an increase of cell lifespan. A synergism between vitamin E and some component(s) in the first of two lots of serum used in the original experiments seems the most likely explanation for our earlier findings.

Author(s): 
Packer, L.
Smith, J. R.
Item Type: 
Journal Article
Publication Title: 
Proceedings of the National Academy of Sciences of the United States of America
Journal Abbreviation: 
Proc. Natl. Acad. Sci. U.S.A.
Publication Date: 
1977-04
Publication Year: 
1977
Pages: 
1640-1641
Volume: 
74
Issue: 
4
ISSN: 
0027-8424
Library Catalog: 
NCBI Published Medical (?)
Extra: 
PMID: 266202 PMCID: PMC430847

Turabian/Chicago Citation

L. Packer and J. R. Smith. 1977-04. "Extension of the lifespan of cultured normal human diploid cells by vitamin E: a reevaluation." Proceedings of the National Academy of Sciences of the United States of America 74: 4: 1640-1641.

Wikipedia Citation

<ref> {{Cite journal | doi = | issn = 0027-8424 | volume = 74 | pages = 1640-1641 | last = Packer | first = L. | coauthors = Smith, J. R. | title = Extension of the lifespan of cultured normal human diploid cells by vitamin E: a reevaluation | journal = Proceedings of the National Academy of Sciences of the United States of America | date = 1977-04 | pmid = | pmc = }} </ref>