Grape extract protects mitochondria from oxidative damage and improves locomotor dysfunction and extends lifespan in a Drosophila Parkinson's disease model
Language: 
English
Abstract: 

A botanical extract (Regrapex-R) prepared from whole grape (Vitis vinifera) and Polygonum cuspidatum, which contains polyphenols, including flavans, anthocyanins, emodin, and resveratrol, exhibited dose-dependent scavenging effects on reactive oxygen species (ROS). The extract inhibited increases of ROS and protein carbonyl in isolated rat liver mitochondria following exposure to 2,2'-azobis (2-amidino propane) dihydrocholoride (AAPH), a potent lipid oxidant generator. The antioxidant effects of this extract were further demonstrated by protecting enzyme activities of the mitochondrial respiratory electron transport chain (complexes I and II) and pyruvate dehydrogenase in isolated liver mitochondria with AAPH insult. In human neuroblastoma cells (SKN-MC), pretreatment of extract protected cells against AAPH induced oxidation in maintaining cell viability and inhibiting excessive ROS generation. Extract was fed to transgenic Drosophila expressing human alpha-synuclein. This model for Parkinson disease recapitulates essential features of the disorder, including loss of dopaminergic neurons in the substantia nigra and a locomotor dysfunction that is displayed by a progressive loss of climbing ability measured using a geotaxis assay. Male transgenic flies fed the extract (0.16-0.64 mg/100 g of culture medium) showed a significant improvement in climbing ability compared to controls. Female transgenic flies showed a significant extension in average lifespan. These results suggest that Regrapex-R is a potent free radical scavenger, a mitochondrial protector, and a candidate for further studies to assess its ability to protect against neurodegenerative disease and potentially extend lifespan.

Author(s): 
Long, Jiangang
Gao, Hongxiang
Sun, Lijuan
Liu, Jiankang
Zhao-Wilson, Xi
Item Type: 
Journal Article
Publication Title: 
Rejuvenation Research
Journal Abbreviation: 
Rejuvenation Res
Publication Date: 
2009-10
Publication Year: 
2009
Pages: 
321-331
Volume: 
12
Issue: 
5
ISSN: 
1557-8577
DOI: 
10.1089/rej.2009.0877
Library Catalog: 
NCBI Published Medical (?)
Extra: 
PMID: 19929256

Turabian/Chicago Citation

Jiangang Long, Hongxiang Gao, Lijuan Sun, Jiankang Liu and Xi Zhao-Wilson. 2009-10. "Grape extract protects mitochondria from oxidative damage and improves locomotor dysfunction and extends lifespan in a Drosophila Parkinson's disease model." Rejuvenation Research 12: 5: 321-331. 10.1089/rej.2009.0877.

Wikipedia Citation

<ref> {{Cite journal | doi = 10.1089/rej.2009.0877 | issn = 1557-8577 | volume = 12 | pages = 321-331 | last = Long | first = Jiangang | coauthors = Gao, Hongxiang, Sun, Lijuan, Liu, Jiankang, Zhao-Wilson, Xi | title = Grape extract protects mitochondria from oxidative damage and improves locomotor dysfunction and extends lifespan in a Drosophila Parkinson's disease model | journal = Rejuvenation Research | date = 2009-10 | pmid = | pmc = }} </ref>