High-performance liquid chromatographic fingerprint for quality control of Terminalia arjuna containing Ayurvedic churna formulation
Language: 
English
Abstract: 

Because Ayurvedic herbal preparations contain a myriad of compounds in complex matrixes, it is difficult to establish quality control standards for raw materials and to standardize finished Ayurvedic drugs. A novel, accurate, and valid fingerprint method was developed using HPLC for quality control of a traditional Ayurvedic Arjuna churna formulation, which is used as a cardiotonic drug. Comprehensive comparison of chromatograms of standardized formulation of Arjuna churna and marketed formulations revealed eight characteristic peaks in chromatograms, which unambiguously confirmed the presence of authentic raw material used in the formulation on the basis of their retention time values and UV data. An HPLC fingerprint was also developed for total sapogenins present in Terminalia arjuna. The six common peaks observed in chromatograms of isolated sapogenins, standardized formulations, and marketed formulations can serve as a quality control tool for qualitative estimation of total saponin glycosides present in an Arjuna churna formulation.

Author(s): 
Chitlange, Sohan S.
Kulkarni, Prajakta S.
Patil, Dada
Patwardhan, Bhushan
Nanda, Rabindra K.
Item Type: 
Journal Article
Publication Title: 
Journal of AOAC International
Journal Abbreviation: 
J AOAC Int
Publication Date: 
2009 Jul-Aug
Publication Year: 
2009
Pages: 
1016-1020
Volume: 
92
Issue: 
4
ISSN: 
1060-3271
Library Catalog: 
NCBI Published Medical (?)
Extra: 
PMID: 19714966

Turabian/Chicago Citation

Sohan S. Chitlange, Prajakta S. Kulkarni, Dada Patil, Bhushan Patwardhan and Rabindra K. Nanda. 2009 Jul-Aug. "High-performance liquid chromatographic fingerprint for quality control of Terminalia arjuna containing Ayurvedic churna formulation." Journal of AOAC International 92: 4: 1016-1020.

Wikipedia Citation

<ref> {{Cite journal | doi = | issn = 1060-3271 | volume = 92 | pages = 1016-1020 | last = Chitlange | first = Sohan S. | coauthors = Kulkarni, Prajakta S., Patil, Dada, Patwardhan, Bhushan, Nanda, Rabindra K. | title = High-performance liquid chromatographic fingerprint for quality control of Terminalia arjuna containing Ayurvedic churna formulation | journal = Journal of AOAC International | date = 2009 Jul-Aug | pmid = | pmc = }} </ref>