Human neural stem cell replacement therapy for amyotrophic lateral sclerosis by spinal transplantation

BACKGROUND: Mutation in the ubiquitously expressed cytoplasmic superoxide dismutase (SOD1) causes an inherited form of Amyotrophic Lateral Sclerosis (ALS). Mutant synthesis in motor neurons drives disease onset and early disease progression. Previous experimental studies have shown that spinal grafting of human fetal spinal neural stem cells (hNSCs) into the lumbar spinal cord of SOD1(G93A) rats leads to a moderate therapeutical effect as evidenced by local ?-motoneuron sparing and extension of lifespan. The aim of the present study was to analyze the degree of therapeutical effect of hNSCs once grafted into the lumbar spinal ventral horn in presymptomatic immunosuppressed SOD1(G93A) rats and to assess the presence and functional integrity of the descending motor system in symptomatic SOD1(G93A) animals. METHODS/PRINCIPAL FINDINGS: Presymptomatic SOD1(G93A) rats (60-65 days old) received spinal lumbar injections of hNSCs. After cell grafting, disease onset, disease progression and lifespan were analyzed. In separate symptomatic SOD1(G93A) rats, the presence and functional conductivity of descending motor tracts (corticospinal and rubrospinal) was analyzed by spinal surface recording electrodes after electrical stimulation of the motor cortex. Silver impregnation of lumbar spinal cord sections and descending motor axon counting in plastic spinal cord sections were used to validate morphologically the integrity of descending motor tracts. Grafting of hNSCs into the lumbar spinal cord of SOD1(G93A) rats protected ?-motoneurons in the vicinity of grafted cells, provided transient functional improvement, but offered no protection to ?-motoneuron pools distant from grafted lumbar segments. Analysis of motor-evoked potentials recorded from the thoracic spinal cord of symptomatic SOD1(G93A) rats showed a near complete loss of descending motor tract conduction, corresponding to a significant (50-65%) loss of large caliber descending motor axons. CONCLUSIONS/SIGNIFICANCE: These data demonstrate that in order to achieve a more clinically-adequate treatment, cell-replacement/gene therapy strategies will likely require both spinal and supraspinal targets.

Hefferan, Michael P.
Galik, Jan
Kakinohana, Osamu
Sekerkova, Gabriela
Santucci, Camila
Marsala, Silvia
Navarro, Roman
Hruska-Plochan, Marian
Johe, Karl
Feldman, Eva
Cleveland, Don W.
Marsala, Martin
Item Type: 
Journal Article
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PloS One
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NCBI Published Medical (?)
PMID: 22916141 PMCID: PMC3423406

Turabian/Chicago Citation

Michael P. Hefferan, Jan Galik, Osamu Kakinohana, Gabriela Sekerkova, Camila Santucci, Silvia Marsala, Roman Navarro, Marian Hruska-Plochan, Karl Johe, Eva Feldman, Don W. Cleveland and Martin Marsala. 2012. "Human neural stem cell replacement therapy for amyotrophic lateral sclerosis by spinal transplantation." PloS One 7: 8: e42614. 10.1371/journal.pone.0042614.

Wikipedia Citation

<ref> {{Cite journal | doi = 10.1371/journal.pone.0042614 | issn = 1932-6203 | volume = 7 | pages = e42614 | last = Hefferan | first = Michael P. | coauthors = Galik, Jan, Kakinohana, Osamu, Sekerkova, Gabriela, Santucci, Camila, Marsala, Silvia, Navarro, Roman, Hruska-Plochan, Marian, Johe, Karl, Feldman, Eva, Cleveland, Don W., Marsala, Martin | title = Human neural stem cell replacement therapy for amyotrophic lateral sclerosis by spinal transplantation | journal = PloS One | date = 2012 | pmid = | pmc = }} </ref>