IL-3 enhances both presentation of exogenous particulate antigen in association with class I major histocompatibility antigen and generation of primary tumor-specific cytolytic T lymphocytes
Language: 
English
Abstract: 

Recent studies have reported that APC can present particulate exogenous Ag in the context of class I MHC to CD8+ CTL, and our laboratory demonstrated that IL-3 could enhance CTL generation to exogenous Ag. In this paper, we wished to determine whether presentation of particulate Ag could be enhanced by IL-3. A T cell hybridoma, B3Z86/90.14 (B3Z) restricted to Ova/Kb, was used as an indicator for presentation of particulate Ag with class I MHC. When activated, this hybridoma expresses lacZ, allowing a simple colorimetric measurement of Ag-specific T cell stimulation. We demonstrated that bone marrow cells stimulated by IL-3 in vivo and in vitro exhibited significantly increased presentation of exogenous OVA linked to beads. Lysate from OVA-transfected line 1 murine lung adenocarcinoma cells (line 1/OVA) was also presented by IL-3-stimulated bone marrow cells, suggesting that these APC can process tumor fragments or debris. Studies using TAP1/2-deficient mice and Ag presentation inhibitors indicate that this exogenous Ag presentation is mediated via the conventional class I MHC pathway. Adoptive transfer of IL-3-stimulated bone marrow cells pulsed with lysate from line 1/OVA tumor cells into naive recipient mice led to the generation of a potent CTL response. These observations indicate that use of such cells may provide a new avenue for development of tumor vaccines.

Author(s): 
Yeh, K. Y.
McAdam, A. J.
Pulaski, B. A.
Shastri, N.
Frelinger, J. G.
Lord, E. M.
Item Type: 
Journal Article
Publication Title: 
Journal of Immunology (Baltimore, Md.: 1950)
Journal Abbreviation: 
J. Immunol.
Publication Date: 
Jun 15, 1998
Publication Year: 
1998
Pages: 
5773-5780
Volume: 
160
Issue: 
12
ISSN: 
0022-1767
Library Catalog: 
NCBI Published Medical (?)
Extra: 
PMID: 9637487

Turabian/Chicago Citation

K. Y. Yeh, A. J. McAdam, B. A. Pulaski, N. Shastri, J. G. Frelinger and E. M. Lord. Jun 15, 1998. "IL-3 enhances both presentation of exogenous particulate antigen in association with class I major histocompatibility antigen and generation of primary tumor-specific cytolytic T lymphocytes." Journal of Immunology (Baltimore, Md.: 1950) 160: 12: 5773-5780.

Wikipedia Citation

<ref> {{Cite journal | doi = | issn = 0022-1767 | volume = 160 | pages = 5773-5780 | last = Yeh | first = K. Y. | coauthors = McAdam, A. J., Pulaski, B. A., Shastri, N., Frelinger, J. G., Lord, E. M. | title = IL-3 enhances both presentation of exogenous particulate antigen in association with class I major histocompatibility antigen and generation of primary tumor-specific cytolytic T lymphocytes | journal = Journal of Immunology (Baltimore, Md.: 1950) | date = Jun 15, 1998 | pmid = | pmc = }} </ref>