Induction of apoptosis by artemisinin relieving the severity of inflammation in caerulein-induced acute pancreatitis
Language: 
English
Abstract: 

AIM: To observe the apoptosis and oncosis of pancreatic acinar cells and secondary inflammatory reaction in pancreatic tissue from rats with acute pancreatitis (AP), and the influences of artemisinin on them. METHODS: AP was induced by 4 intraperitoneal injections of caerulein at 1 h intervals. To induce apoptosis, solution of artemisinin (50 mg/kg) was given intraperitoneally 1, 12, 24 and 36 h after the last caerulein injection. Histological examination of impairment of pancreatic tissue and detection of serum amylase were performed to evaluate the severity of acute pancreatitis. Apoptosis and oncosis were detected with acridine orange (AO) and ethylene dibromide (EB) staining. Caspase-3 and myeloperoxidase (MPO) activity were measured by colorimetric assay. Nuclear factor-kappa B (NF-kappaB) activation was detected by flow cytometry. Macrophage inflammatory protein-1alpha (MIP-1alpha) protein was measured by Western blot. Interleukin-1beta (IL-1beta) mRNA was detected by RT-PCR. RESULTS: Addition of artemisinin increased the number of apoptotic cells (11.7% +/- 1.4% vs 6.3% +/- 0.7%, P < 0.05), while reduced the number of oncotic cells (13.0% +/- 2.4% vs 17.5% +/- 2.2%, P < 0.05). The activity of caspase-3 speeded up (1.52 +/- 0.21 vs 1.03 +/- 0.08, P < 0.05), the pancreas pathological impairment was relieved (3.0 +/- 0.5 vs 4.0 +/- 0.5, P < 0.05) and the level of serum amylase decreased (5642 +/- 721 U/dL vs 7821 +/- 653 U/dL, P < 0.05). The activation of NF-kB (29% +/- 4.1% vs 42% +/- 5.8%), MIP-1alpha protein (3.7 +/- 0.5 vs 5.8 +/- 0.7), MPO (0.52 +/- 0.06 U/g vs 0.68 +/- 0.09 U/g), IL-1beta mRNA (1.7 +/- 0.3 vs 2.4 +/- 0.4) in the apoptosis inducing group was obviously decreased (P < 0.05). CONCLUSION: Inducing apoptosis can relieve pathological impairment and inflammatory reaction in AP rats.

Author(s): 
Zhao, Ming
Xue, Dong-Bo
Zheng, Biao
Zhang, Wei-Hui
Pan, Shang-ha
Sun, Bei
Item Type: 
Journal Article
Publication Title: 
World journal of gastroenterology: WJG
Journal Abbreviation: 
World J. Gastroenterol.
Publication Date: 
11/14/2007
Publication Year: 
2007
Pages: 
5612-5617
Volume: 
13
Issue: 
42
ISSN: 
1007-9327
Library Catalog: 
PubMed
Extra: 
PMID: 17948936 PMCID: PMC4172741

Turabian/Chicago Citation

Ming Zhao, Dong-Bo Xue, Biao Zheng, Wei-Hui Zhang, Shang-ha Pan and Bei Sun. 11/14/2007. "Induction of apoptosis by artemisinin relieving the severity of inflammation in caerulein-induced acute pancreatitis." World journal of gastroenterology: WJG 13: 42: 5612-5617.

Wikipedia Citation

<ref> {{Cite journal | doi = | issn = 1007-9327 | volume = 13 | pages = 5612-5617 | last = Zhao | first = Ming | coauthors = Xue, Dong-Bo, Zheng, Biao, Zhang, Wei-Hui, Pan, Shang-ha, Sun, Bei | title = Induction of apoptosis by artemisinin relieving the severity of inflammation in caerulein-induced acute pancreatitis | journal = World journal of gastroenterology: WJG | date = 11/14/2007 | pmid = | pmc = }} </ref>