Inhibition of inducible nitric oxide synthesis by the herbal preparation Padma 28 in macrophage cell line
Language: 
English
Abstract: 

Padma 28 is a mixture of herbs used in traditional Tibetan medicine with anti-inflammatory activities. We investigated the effects of Padma 28 on nitric oxide (NO) production by the inducible nitric oxide synthase (iNOS) in lipopolysaccharide stimulated mouse macrophages (RAW 264.7). Padma 28 (0-900 microg/mL) induced a concentration dependent inhibition of inducible nitric oxide synthesis. iNOS protein expression showed a concentration dependent reduction as revealed by immunoblotting when cells were incubated with increasing amounts of Padma 28. Padma 28 decreased iNOS mRNA levels as shown by RT-PCR. Aqueous extracts from costi amari radix (costus root, the dried root of Saussurea lappa) and the outer cover of myrobalani fructus (the dried fruit of Terminalia chebula), constituents of the complex herb preparation Padma 28, were found to inhibit inducible nitric oxide synthesis by decreasing iNOS protein and iNOS mRNA levels. The inhibition of inducible nitric oxide synthesis might contribute to the anti-inflammatory activities of Padma 28.

Author(s): 
Moeslinger, T.
Friedl, R.
Volf, I.
Brunner, M.
Koller, E.
Spieckermann, P. G.
Item Type: 
Journal Article
Publication Title: 
Canadian Journal of Physiology and Pharmacology
Journal Abbreviation: 
Can. J. Physiol. Pharmacol.
Publication Date: 
2000-11
Publication Year: 
2000
Pages: 
861-866
Volume: 
78
Issue: 
11
ISSN: 
0008-4212
Library Catalog: 
PubMed
Extra: 
PMID: 11100933

Turabian/Chicago Citation

T. Moeslinger, R. Friedl, I. Volf, M. Brunner, E. Koller and P. G. Spieckermann. 2000-11. "Inhibition of inducible nitric oxide synthesis by the herbal preparation Padma 28 in macrophage cell line." Canadian Journal of Physiology and Pharmacology 78: 11: 861-866.

Wikipedia Citation

<ref> {{Cite journal | doi = | issn = 0008-4212 | volume = 78 | pages = 861-866 | last = Moeslinger | first = T. | coauthors = Friedl, R., Volf, I., Brunner, M., Koller, E., Spieckermann, P. G. | title = Inhibition of inducible nitric oxide synthesis by the herbal preparation Padma 28 in macrophage cell line | journal = Canadian Journal of Physiology and Pharmacology | date = 2000-11 | pmid = | pmc = }} </ref>