An investigation of the effects of the core protein telomerase reverse transcriptase on Wnt signaling in breast cancer cells
Language: 
English
Abstract: 

Telomerase canonically maintains telomeres, but recent reports have suggested that the core protein mammalian telomerase reverse transcriptase (TERT) component, together with the chromatin remodeling factor BRG1 and ?-catenin, may also bind to and promote expression of Wnt target genes. However, this proposed noncanonical role of TERT in Wnt signaling has been controversial. Here, we investigated the effects of human TERT (hTERT) on Wnt signaling in human breast cancer lines and HeLa cells. We failed to find evidence for physical association of hTERT with BRG1 or ?-catenin; instead, we present evidence that anti-FLAG antibody cross-reactivity properties may explain the previously reported interaction of hTERT with ?-catenin. Furthermore, altering hTERT levels in four different breast cancer cell lines caused minimal and discordant effects on Wnt target and Wnt pathway gene expression. Although hTERT's role in Wnt signaling was addressed only indirectly, no significant representation of Wnt target genes was detected in chromatin immunoprecipitation-sequencing (ChIP-seq) and chromatin isolation by RNA purification and sequencing (ChIRP-seq) loci cooccupied in HeLa S3 cells by both BRG1 and hTR. In summary, our evidence fails to support the idea of a biologically consistent hTERT interaction with the Wnt pathway in human breast cancer cells, and any detectable influence of hTERT depended on cell type and experimental system.

Author(s): 
Listerman, Imke
Gazzaniga, Francesca S.
Blackburn, Elizabeth H.
Item Type: 
Journal Article
Publication Title: 
Molecular and Cellular Biology
Journal Abbreviation: 
Mol. Cell. Biol.
Publication Date: 
2014-01
Publication Year: 
2014
Pages: 
280-289
Volume: 
34
Issue: 
2
ISSN: 
1098-5549
DOI: 
10.1128/MCB.00844-13
Library Catalog: 
NCBI Published Medical (?)
Extra: 
PMID: 24216762 PMCID: PMC3911296

Turabian/Chicago Citation

Imke Listerman, Francesca S. Gazzaniga and Elizabeth H. Blackburn. 2014-01. "An investigation of the effects of the core protein telomerase reverse transcriptase on Wnt signaling in breast cancer cells." Molecular and Cellular Biology 34: 2: 280-289. 10.1128/MCB.00844-13.

Wikipedia Citation

<ref> {{Cite journal | doi = 10.1128/MCB.00844-13 | issn = 1098-5549 | volume = 34 | pages = 280-289 | last = Listerman | first = Imke | coauthors = Gazzaniga, Francesca S., Blackburn, Elizabeth H. | title = An investigation of the effects of the core protein telomerase reverse transcriptase on Wnt signaling in breast cancer cells | journal = Molecular and Cellular Biology | date = 2014-01 | pmid = | pmc = }} </ref>