Isolation of radiolabeled isoflavones from kudzu (Pueraria lobata) root cultures
Language: 
English
Abstract: 

Isoflavones have potential for preventing and treating several chronic health conditions, such as osteoporosis, cardiovascular disease, and cancer. In this study, radiolabeled isoflavones were recovered from kudzu (Pueraria lobata) root cultures after incubation with uniformly labeled (14)C-sucrose in the culture medium for 21 days. Approximately 19% of administered label was recovered in the isoflavone-rich dried extracts of kudzu root cultures (90.2 microCi/g or 3.3 MBq/g extract). HPLC-PDA analysis revealed the predominant isoflavones isolated from kudzu root cultures to be puerarin, daidzin, and malonyl-daidzin. The average concentration of the major isoflavone puerarin in kudzu root cultures was 33.6 mg/g extract, with a specific activity of 63.5 microCi/g (2.3 MBq/g). The isolated isoflavones were sufficiently (14)C-labeled to permit utilization for subsequent in vivo metabolic tracking studies.

Author(s): 
Reppert, Adam
Yousef, Gad G.
Rogers, Randy B.
Lila, Mary Ann
Item Type: 
Journal Article
Publication Title: 
Journal of Agricultural and Food Chemistry
Journal Abbreviation: 
J. Agric. Food Chem.
Publication Date: 
Sep 10, 2008
Publication Year: 
2008
Pages: 
7860-7865
Volume: 
56
Issue: 
17
ISSN: 
1520-5118
DOI: 
10.1021/jf801413z
Library Catalog: 
NCBI Published Medical (?)
Extra: 
PMID: 18690681 PMCID: PMC2676909

Turabian/Chicago Citation

Adam Reppert, Gad G. Yousef, Randy B. Rogers and Mary Ann Lila. Sep 10, 2008. "Isolation of radiolabeled isoflavones from kudzu (Pueraria lobata) root cultures." Journal of Agricultural and Food Chemistry 56: 17: 7860-7865. 10.1021/jf801413z.

Wikipedia Citation

<ref> {{Cite journal | doi = 10.1021/jf801413z | issn = 1520-5118 | volume = 56 | pages = 7860-7865 | last = Reppert | first = Adam | coauthors = Yousef, Gad G., Rogers, Randy B., Lila, Mary Ann | title = Isolation of radiolabeled isoflavones from kudzu (Pueraria lobata) root cultures | journal = Journal of Agricultural and Food Chemistry | date = Sep 10, 2008 | pmid = | pmc = }} </ref>