Loss of Drosophila Ataxin-7, a SAGA subunit, reduces H2B ubiquitination and leads to neural and retinal degeneration
Language: 
English
Abstract: 

The Spt-Ada-Gcn5-acetyltransferase (SAGA) chromatin-modifying complex possesses acetyltransferase and deubiquitinase activities. Within this modular complex, Ataxin-7 anchors the deubiquitinase activity to the larger complex. Here we identified and characterized Drosophila Ataxin-7 and found that reduction of Ataxin-7 protein results in loss of components from the SAGA complex. In contrast to yeast, where loss of Ataxin-7 inactivates the deubiquitinase and results in increased H2B ubiquitination, loss of Ataxin-7 results in decreased H2B ubiquitination and H3K9 acetylation without affecting other histone marks. Interestingly, the effect on ubiquitination was conserved in human cells, suggesting a novel mechanism regulating histone deubiquitination in higher organisms. Consistent with this mechanism in vivo, we found that a recombinant deubiquitinase module is active in the absence of Ataxin-7 in vitro. When we examined the consequences of reduced Ataxin-7 in vivo, we found that flies exhibited pronounced neural and retinal degeneration, impaired movement, and early lethality.

Author(s): 
Mohan, Ryan D.
Dialynas, George
Weake, Vikki M.
Liu, Jianqi
Martin-Brown, Skylar
Florens, Laurence
Washburn, Michael P.
Workman, Jerry L.
Abmayr, Susan M.
Item Type: 
Journal Article
Publication Title: 
Genes & Development
Journal Abbreviation: 
Genes Dev.
Publication Date: 
2/1/2014
Publication Year: 
2014
Pages: 
259-272
Volume: 
28
Issue: 
3
ISSN: 
1549-5477
DOI: 
10.1101/gad.225151.113
Library Catalog: 
NCBI Published Medical (?)
Extra: 
PMID: 24493646 PMCID: PMC3923968

Turabian/Chicago Citation

Ryan D. Mohan, George Dialynas, Vikki M. Weake, Jianqi Liu, Skylar Martin-Brown, Laurence Florens, Michael P. Washburn, Jerry L. Workman and Susan M. Abmayr. 2/1/2014. "Loss of Drosophila Ataxin-7, a SAGA subunit, reduces H2B ubiquitination and leads to neural and retinal degeneration." Genes & Development 28: 3: 259-272. 10.1101/gad.225151.113.

Wikipedia Citation

<ref> {{Cite journal | doi = 10.1101/gad.225151.113 | issn = 1549-5477 | volume = 28 | pages = 259-272 | last = Mohan | first = Ryan D. | coauthors = Dialynas, George, Weake, Vikki M., Liu, Jianqi, Martin-Brown, Skylar, Florens, Laurence, Washburn, Michael P., Workman, Jerry L., Abmayr, Susan M. | title = Loss of Drosophila Ataxin-7, a SAGA subunit, reduces H2B ubiquitination and leads to neural and retinal degeneration | journal = Genes & Development | date = 2/1/2014 | pmid = | pmc = }} </ref>