Low oxygen tension alleviates oxidative damage and delays cellular senescence in G6PD-deficient cells
Language: 
English
Abstract: 

Previous studies have shown that glucose-6-phosphate dehydrogenase (G6PD)-deficient cells are under increased oxidative stress and undergo premature cellular senescence. The present study demonstrates that G6PD-deficient cells cultured under 3% oxygen concentration had an extended replicative lifespan, as compared with those cultured under atmospheric oxygen level. This was accompanied by a reduction in the number of senescence-associated beta-galactosidase (SA-beta-Gal) positive and morphologically senile cells at comparable population doubling levels (PDL). Concomitant with the extension of lifespan was decreased production of reactive oxygen species. Additionally, lifespan extension was paralleled by the greatly abated formation of such oxidative damage markers as 8-hydroxy-deoxyguanosine (8-OHdG) as well as the oxidized and cross-linked proteins. Moreover, the mitochondrial mass increased, but the mitochondrial membrane potential DeltaPsim decreased in cells upon serial propagation. These changes were inhibited by lowering the oxygen tension. Our findings provide additional support to the notion that oxidative damage contributes to replicative senescence of G6PD-deficient cells and reduction of oxidative damage by lowering oxygen tension can delay the onset of cellular senescence.

Author(s): 
Ho, Hung-Yao
Cheng, Mei-Ling
Cheng, Pei-Fen
Chiu, Daniel Tsun-Yee
Item Type: 
Journal Article
Publication Title: 
Free Radical Research
Journal Abbreviation: 
Free Radic. Res.
Publication Date: 
2007-05
Publication Year: 
2007
Pages: 
571-579
Volume: 
41
Issue: 
5
ISSN: 
1071-5762
DOI: 
10.1080/10715760601184819
Library Catalog: 
NCBI Published Medical (?)
Extra: 
PMID: 17454140

Turabian/Chicago Citation

Hung-Yao Ho, Mei-Ling Cheng, Pei-Fen Cheng and Daniel Tsun-Yee Chiu. 2007-05. "Low oxygen tension alleviates oxidative damage and delays cellular senescence in G6PD-deficient cells." Free Radical Research 41: 5: 571-579. 10.1080/10715760601184819.

Wikipedia Citation

<ref> {{Cite journal | doi = 10.1080/10715760601184819 | issn = 1071-5762 | volume = 41 | pages = 571-579 | last = Ho | first = Hung-Yao | coauthors = Cheng, Mei-Ling, Cheng, Pei-Fen, Chiu, Daniel Tsun-Yee | title = Low oxygen tension alleviates oxidative damage and delays cellular senescence in G6PD-deficient cells | journal = Free Radical Research | date = 2007-05 | pmid = | pmc = }} </ref>