Metagenomic pyrosequencing and microbial identification
Language: 
English
Abstract: 

BACKGROUND: The Human Microbiome Project has ushered in a new era for human metagenomics and high-throughput next-generation sequencing strategies. CONTENT: This review describes evolving strategies in metagenomics, with a special emphasis on the core technology of DNA pyrosequencing. The challenges of microbial identification in the context of microbial populations are discussed. The development of next-generation pyrosequencing strategies and the technical hurdles confronting these methodologies are addressed. Bioinformatics-related topics include taxonomic systems, sequence databases, sequence-alignment tools, and classifiers. DNA sequencing based on 16S rRNA genes or entire genomes is summarized with respect to potential pyrosequencing applications. SUMMARY: Both the approach of 16S rDNA amplicon sequencing and the whole-genome sequencing approach may be useful for human metagenomics, and numerous bioinformatics tools are being deployed to tackle such vast amounts of microbiological sequence diversity. Metagenomics, or genetic studies of microbial communities, may ultimately contribute to a more comprehensive understanding of human health, disease susceptibilities, and the pathophysiology of infectious and immune-mediated diseases.

Author(s): 
Petrosino, Joseph F.
Highlander, Sarah
Luna, Ruth Ann
Gibbs, Richard A.
Versalovic, James
Item Type: 
Journal Article
Publication Title: 
Clinical Chemistry
Journal Abbreviation: 
Clin. Chem.
Publication Date: 
May 2009
Publication Year: 
2009
Pages: 
856-866
Volume: 
55
Issue: 
5
ISSN: 
1530-8561
DOI: 
10.1373/clinchem.2008.107565
Library Catalog: 
NCBI Published Medical (?)
Extra: 
PMID: 19264858 PMCID: PMC2892905

Turabian/Chicago Citation

Joseph F. Petrosino, Sarah Highlander, Ruth Ann Luna, Richard A. Gibbs and James Versalovic. May 2009. "Metagenomic pyrosequencing and microbial identification." Clinical Chemistry 55: 5: 856-866. 10.1373/clinchem.2008.107565.

Wikipedia Citation

<ref> {{Cite journal | doi = 10.1373/clinchem.2008.107565 | issn = 1530-8561 | volume = 55 | pages = 856-866 | last = Petrosino | first = Joseph F. | coauthors = Highlander, Sarah, Luna, Ruth Ann, Gibbs, Richard A., Versalovic, James | title = Metagenomic pyrosequencing and microbial identification | journal = Clinical Chemistry | date = May 2009 | pmid = | pmc = }} </ref>