MR contrast probes that trace gene transcripts for cerebral ischemia in live animals
Language: 
English
Abstract: 

The aim of this research was to validate transcription magnetic resonance (MR) imaging (MRI) for gene transcript targeting in acute neurological disorders in live subjects. We delivered three MR probe variants with superparamagnetic iron oxide nanoparticles (SPION, a T2 susceptibility agent) linked to a phosphorothioate-modified oligodeoxynucleotide (sODN) complementary to c-fos mRNA (SPION-cfos) or beta-actin mRNA (SPION-beta-actin) and to sODN with random sequence (SPION-Ran). Each probe (1 microg Fe in 2 microl) was delivered via intracerebroventricular infusion to the left cerebral ventricle of male C57Black6 mice. We demonstrated SPION retention, measured as decreased T2* signal or increased R2* value (R2* = 1/T2*). Animals that received the SPION-beta-actin probe exhibited the highest R2* values, followed (in descending order) by SPION-cfos and SPION-Ran. SPION-cfos retention was localized in brain regions where SPION-cfos was present and where hybrids of SPION-cfos and its target c-fos mRNA were detected by in situ reverse transcription PCR. In animals that experienced cerebral ischemia, SPION-cfos retention was significantly increased in locations where c-fos mRNA increased in response to the ischemic insult; these elevations were not observed for SPION-beta-actin and SPION-Ran. This study should enable MR detection of mRNA alteration in disease models of the central nervous system.

Author(s): 
Liu, Christina H.
Huang, Shuning
Cui, Jiankun
Kim, Young R.
Farrar, Christian T.
Moskowitz, Michael A.
Rosen, Bruce R.
Liu, Philip K.
Item Type: 
Journal Article
Publication Title: 
FASEB journal: official publication of the Federation of American Societies for Experimental Biology
Journal Abbreviation: 
FASEB J.
Publication Date: 
Sep 2007
Publication Year: 
2007
Pages: 
3004-3015
Volume: 
21
Issue: 
11
ISSN: 
1530-6860
DOI: 
10.1096/fj.07-8203com
Library Catalog: 
NCBI Published Medical (?)
Extra: 
PMID: 17478745 PMCID: PMC2657320

Turabian/Chicago Citation

Christina H. Liu, Shuning Huang, Jiankun Cui, Young R. Kim, Christian T. Farrar, Michael A. Moskowitz, Bruce R. Rosen and Philip K. Liu. Sep 2007. "MR contrast probes that trace gene transcripts for cerebral ischemia in live animals." FASEB journal: official publication of the Federation of American Societies for Experimental Biology 21: 11: 3004-3015. 10.1096/fj.07-8203com.

Wikipedia Citation

<ref> {{Cite journal | doi = 10.1096/fj.07-8203com | issn = 1530-6860 | volume = 21 | pages = 3004-3015 | last = Liu | first = Christina H. | coauthors = Huang, Shuning, Cui, Jiankun, Kim, Young R., Farrar, Christian T., Moskowitz, Michael A., Rosen, Bruce R., Liu, Philip K. | title = MR contrast probes that trace gene transcripts for cerebral ischemia in live animals | journal = FASEB journal: official publication of the Federation of American Societies for Experimental Biology | date = Sep 2007 | pmid = | pmc = }} </ref>