N-acetylglucosamine prevents IL-1 beta-mediated activation of human chondrocytes
Language: 
English
Abstract: 

Glucosamine represents one of the most commonly used drugs to treat osteoarthritis. However, mechanisms of its antiarthritic activities are still poorly understood. The present study identifies a novel mechanism of glucosamine-mediated anti-inflammatory activity. It is shown that both glucosamine and N-acetylglucosamine inhibit IL-1beta- and TNF-alpha-induced NO production in normal human articular chondrocytes. The effect of the sugars on NO production is specific, since several other monosaccharides, including glucose, glucuronic acid, and N-acetylmannosamine, do not express this activity. Furthermore, N-acetylglucosamine polymers, including the dimer and the trimer, also do not affect NO production. The observed suppression of IL-1beta-induced NO production is associated with inhibition of inducible NO synthase mRNA and protein expression. In addition, N-acetylglucosamine also suppresses the production of IL-1beta-induced cyclooxygenase-2 and IL-6. The constitutively expressed cyclooxygenase-1, however, was not affected by the sugar. N-acetylglucosamine-mediated inhibition of the IL-1beta response of human chondrocytes was not associated with the decreased inhibition of the mitogen-activated protein kinases c-Jun N-terminal kinase, extracellular signal-related kinase, and p38, nor with activation of the transcription factor NF-kappaB. In conclusion, these results demonstrate that N-acetylglucosamine expresses a unique range of activities and identifies a novel mechanism for the inhibition of inflammatory processes.

Author(s): 
Shikhman, A. R.
Kuhn, K.
Alaaeddine, N.
Lotz, M.
Item Type: 
Journal Article
Publication Title: 
Journal of Immunology (Baltimore, Md.: 1950)
Journal Abbreviation: 
J. Immunol.
Publication Date: 
Apr 15, 2001
Publication Year: 
2001
Pages: 
5155-5160
Volume: 
166
Issue: 
8
ISSN: 
0022-1767
Library Catalog: 
NCBI Published Medical (?)
Extra: 
PMID: 11290798

Turabian/Chicago Citation

A. R. Shikhman, K. Kuhn, N. Alaaeddine and M. Lotz. Apr 15, 2001. "N-acetylglucosamine prevents IL-1 beta-mediated activation of human chondrocytes." Journal of Immunology (Baltimore, Md.: 1950) 166: 8: 5155-5160.

Wikipedia Citation

<ref> {{Cite journal | doi = | issn = 0022-1767 | volume = 166 | pages = 5155-5160 | last = Shikhman | first = A. R. | coauthors = Kuhn, K., Alaaeddine, N., Lotz, M. | title = N-acetylglucosamine prevents IL-1 beta-mediated activation of human chondrocytes | journal = Journal of Immunology (Baltimore, Md.: 1950) | date = Apr 15, 2001 | pmid = | pmc = }} </ref>