NK cell maturation and function in C57BL/6 mice are altered by caloric restriction
Language: 
English
Abstract: 

NK cells are a heterogenous population of innate lymphocytes with diverse functional attributes critical for early protection from viral infections. We have previously reported a decrease in influenza-induced NK cell cytotoxicity in 6-mo-old C57BL/6 calorically restricted (CR) mice. In the current study, we extend our findings on the influence of CR on NK cell phenotype and function in the absence of infection. We demonstrate that reduced mature NK cell subsets result in increased frequencies of CD127(+) NK cells in CR mice, skewing the function of the total NK cell pool. NK cells from CR mice produced TNF-α and GM-CSF at a higher level, whereas IFN-γ production was impaired following IL-2 plus IL-12 or anti-NK1.1 stimulation. NK cells from CR mice were highly responsive to stimulation with YAC-1 cells such that CD27(-)CD11b(+) NK cells from CR mice produced granzyme B and degranulated at a higher frequency than CD27(-)CD11b(+) NK cells from ad libitum fed mice. CR has been shown to be a potent dietary intervention, yet the mechanisms by which the CR increases life span have yet to be fully understood. To our knowledge, these findings are the first in-depth analysis of the effects of caloric intake on NK cell phenotype and function and provide important implications regarding potential ways in which CR alters NK cell function prior to infection or cancer.

Author(s): 
Clinthorne, Jonathan F.
Beli, Eleni
Duriancik, David M.
Gardner, Elizabeth M.
Item Type: 
Journal Article
Publication Title: 
Journal of Immunology (Baltimore, Md.: 1950)
Journal Abbreviation: 
J. Immunol.
Publication Date: 
Jan 15, 2013
Publication Year: 
2013
Pages: 
712-722
Volume: 
190
Issue: 
2
ISSN: 
1550-6606
DOI: 
10.4049/jimmunol.1201837
Library Catalog: 
NCBI Published Medical (?)
Extra: 
PMID: 23241894 PMCID: PMC4080414

Turabian/Chicago Citation

Jonathan F. Clinthorne, Eleni Beli, David M. Duriancik and Elizabeth M. Gardner. Jan 15, 2013. "NK cell maturation and function in C57BL/6 mice are altered by caloric restriction." Journal of Immunology (Baltimore, Md.: 1950) 190: 2: 712-722. 10.4049/jimmunol.1201837.

Wikipedia Citation

<ref> {{Cite journal | doi = 10.4049/jimmunol.1201837 | issn = 1550-6606 | volume = 190 | pages = 712-722 | last = Clinthorne | first = Jonathan F. | coauthors = Beli, Eleni, Duriancik, David M., Gardner, Elizabeth M. | title = NK cell maturation and function in C57BL/6 mice are altered by caloric restriction | journal = Journal of Immunology (Baltimore, Md.: 1950) | date = Jan 15, 2013 | pmid = | pmc = }} </ref>