A novel DNA deletion-ligation reaction catalyzed in vitro by a developmentally controlled activity from Tetrahymena cells
Language: 
English
Abstract: 

Developmentally controlled genomic deletion-ligations occur during ciliate macronuclear differentiation. We have identified a novel activity in Tetrahymena cell-free extracts that efficiently catalyzes a specific set of intramolecular DNA deletion-ligation reactions. When synthetic DNA oligonucleotide substrates were used, all the deletion-ligation products resembled those formed in vivo in that they resulted from deletions between pairs of short direct repeats. The reaction is ATP-dependent, salt-sensitive, and strongly influenced by the oligonucleotide substrate sequence. The deletion-ligation activity has an apparent size of 200-500 kd, no nuclease-sensitive component, and is highly enriched in cells developing new macronuclei. The temperature inactivation profile of the activity parallels the temperature lethality profile specific for Tetrahymena cells developing new macronuclei. We suggest that this deletion-ligation activity carries out the genomic deletions in developing macronuclei in vivo.

Author(s): 
Robinson, E. K.
Cohen, P. D.
Blackburn, E. H.
Item Type: 
Journal Article
Publication Title: 
Cell
Journal Abbreviation: 
Cell
Publication Date: 
9/8/1989
Publication Year: 
1989
Pages: 
887-900
Volume: 
58
Issue: 
5
ISSN: 
0092-8674
Library Catalog: 
NCBI Published Medical (?)
Extra: 
PMID: 2776215

Turabian/Chicago Citation

E. K. Robinson, P. D. Cohen and E. H. Blackburn. 9/8/1989. "A novel DNA deletion-ligation reaction catalyzed in vitro by a developmentally controlled activity from Tetrahymena cells." Cell 58: 5: 887-900.

Wikipedia Citation

<ref> {{Cite journal | doi = | issn = 0092-8674 | volume = 58 | pages = 887-900 | last = Robinson | first = E. K. | coauthors = Cohen, P. D., Blackburn, E. H. | title = A novel DNA deletion-ligation reaction catalyzed in vitro by a developmentally controlled activity from Tetrahymena cells | journal = Cell | date = 9/8/1989 | pmid = | pmc = }} </ref>