Ophiopogonin B-induced autophagy in non-small cell lung cancer cells via inhibition of the PI3K/Akt signaling pathway
Language: 
English
Abstract: 

Ophiopogonin B (OP-B) is a bioactive component of Radix Ophiopogon Japonicus, which is often used in Chinese traditional medicine to treat pulmonary disease. However, whether or not OP-B has any potential antitumor activity has not been reported. Here, we show that the non-small cell lung cancer (NSCLC) cell lines NCI-H157 and NCI-H460 treated with OP-B grow more slowly and accumulate vacuoles in their cytoplasm compared to untreated control cells. Flow cytometric analysis showed that the cells were arrested in G0/G1 phase. Nuclear morphology, Annexin-V/PI staining, and expression of cleaved caspase-3 all confirm that OP-B does not induce apoptosis. Instead, based on results from both transmission electron microscopy (TEM) and the expression of microtubule-associated protein 1 light chain 3-II (LC3-II), we determined that OP-B treatment induced autophagy in both cell lines. Next, we examined the PI3K/Akt/mTOR signaling pathway and found that OP-B inhibited phosphorylation of Akt (Ser473, Thr308) in NCI-H157 cells and also inhibited several key components of the pathway in NCI-H460 cells, such as p-Akt(Ser473, Thr308), p-p70S6K (Thr389). Additionally, insulin-mediated activation of the PI3K/Akt/mTOR pathway provides evidence that activation of this pathway may correlate with induction of autophagy in H460 cells. Therefore, OP-B is a prospective inhibitor of PI3K/Akt and may be used as an alternative compound to treat NSCLC.

Author(s): 
Chen, Meijuan
Du, Yuhong
Qui, Min
Wang, Mingyan
Chen, Kejun
Huang, Zhenzhou
Jiang, Miao
Xiong, Fei
Chen, Jianping
Zhou, Jing
Jiang, Fengrong
Yin, Lian
Tang, Yuping
Ye, Lihong
Zhan, Zhen
Duan, Jin-Αo
Fu, Hai-Αn
Zhang, Xu
Item Type: 
Journal Article
Publication Title: 
Oncology Reports
Journal Abbreviation: 
Oncol. Rep.
Publication Date: 
Feb 2013
Publication Year: 
2013
Pages: 
430-436
Volume: 
29
Issue: 
2
ISSN: 
1791-2431
DOI: 
10.3892/or.2012.2131
Library Catalog: 
NCBI Published Medical (?)
Extra: 
PMID: 23151908 PMCID: PMC3583607

Turabian/Chicago Citation

Meijuan Chen, Yuhong Du, Min Qui, Mingyan Wang, Kejun Chen, Zhenzhou Huang, Miao Jiang, Fei Xiong, Jianping Chen, Jing Zhou, Fengrong Jiang, Lian Yin, Yuping Tang, Lihong Ye, Zhen Zhan, Jin-Αo Duan, Hai-Αn Fu and Xu Zhang. Feb 2013. "Ophiopogonin B-induced autophagy in non-small cell lung cancer cells via inhibition of the PI3K/Akt signaling pathway." Oncology Reports 29: 2: 430-436. 10.3892/or.2012.2131.

Wikipedia Citation

<ref> {{Cite journal | doi = 10.3892/or.2012.2131 | issn = 1791-2431 | volume = 29 | pages = 430-436 | last = Chen | first = Meijuan | coauthors = Du, Yuhong, Qui, Min, Wang, Mingyan, Chen, Kejun, Huang, Zhenzhou, Jiang, Miao, Xiong, Fei, Chen, Jianping, Zhou, Jing, Jiang, Fengrong, Yin, Lian, Tang, Yuping, Ye, Lihong, Zhan, Zhen, Duan, Jin-Αo, Fu, Hai-Αn, Zhang, Xu | title = Ophiopogonin B-induced autophagy in non-small cell lung cancer cells via inhibition of the PI3K/Akt signaling pathway | journal = Oncology Reports | date = Feb 2013 | pmid = | pmc = }} </ref>