Pharmacological modulation of histone demethylase activity by a small molecule isolated from subcritical water extracts of Sasa senanensis leaves prolongs the lifespan of Drosophila melanogaster
Language: 
English
Abstract: 

BACKGROUND: Extracts of Sasa senanensis Rehder are used in traditional Japanese medicine; however, little is known about the underlying mechanisms of their potential health benefits. METHODS: S. senanensis leaves were extracted with subcritical water. An active small-molecule was isolated using reversed-phase high-performance liquid chromatography (HPLC), and identified as 3,4-dihydroxybenzaldehyde (protocatechuic aldehyde or PA). The effects of PA on the activity of histone demethylase, the Drosophila melanogaster lifespan and gene expression in Drosophila S2 cells were investigated. RESULTS: PA inhibited the activity of Jumonji domain-containing protein 2A (JMJD2A) histone demethylase in a dose-dependent manner with a half-maximal inhibitory concentration (IC50) of 11.6 ?M. However, there was no effect on lysine-specific demethylase 1 (LSD1), histone deacetylase 1 (HDAC1) or HDAC8. PA significantly extended the lifespan of female, but not male, Drosophila. In Drosophila S2 cells, the eukaryotic translation initiation factor 4E binding protein (4E-BP) was up-regulated by PA exposure. CONCLUSIONS: Our findings provide insight into the possible relationship between the pharmacological modulation of histone demethylation and lifespan extension by PA; they might also be important in the development of alternative therapies for age-related disorders.

Author(s): 
Nakagawa-Yagi, Yuzo
Sato, Yukiko
Matsumoto, Emi
Nakatsuka, Shin-Ichi
Sakaki, Tsuyoshi
Muramatsu, Yukiko
Hara, Takaaki
Aigaki, Toshiro
Item Type: 
Journal Article
Publication Title: 
BMC complementary and alternative medicine
Journal Abbreviation: 
BMC Complement Altern Med
Publication Date: 
2012
Publication Year: 
2012
Pages: 
101
Volume: 
12
ISSN: 
1472-6882
DOI: 
10.1186/1472-6882-12-101
Library Catalog: 
NCBI Published Medical (?)
Extra: 
PMID: 22809229 PMCID: PMC3551771

Turabian/Chicago Citation

Yuzo Nakagawa-Yagi, Yukiko Sato, Emi Matsumoto, Shin-Ichi Nakatsuka, Tsuyoshi Sakaki, Yukiko Muramatsu, Takaaki Hara and Toshiro Aigaki. 2012. "Pharmacological modulation of histone demethylase activity by a small molecule isolated from subcritical water extracts of Sasa senanensis leaves prolongs the lifespan of Drosophila melanogaster." BMC complementary and alternative medicine 12: 101. 10.1186/1472-6882-12-101.

Wikipedia Citation

<ref> {{Cite journal | doi = 10.1186/1472-6882-12-101 | issn = 1472-6882 | volume = 12 | pages = 101 | last = Nakagawa-Yagi | first = Yuzo | coauthors = Sato, Yukiko, Matsumoto, Emi, Nakatsuka, Shin-Ichi, Sakaki, Tsuyoshi, Muramatsu, Yukiko, Hara, Takaaki, Aigaki, Toshiro | title = Pharmacological modulation of histone demethylase activity by a small molecule isolated from subcritical water extracts of Sasa senanensis leaves prolongs the lifespan of Drosophila melanogaster | journal = BMC complementary and alternative medicine | date = 2012 | pmid = | pmc = }} </ref>