Physiological responses and differential gene expression in Prunus rootstocks under iron deficiency conditions
Language: 
English
Abstract: 

Two Prunus rootstocks, the Myrobalan plum P 2175 and the interspecific peach-almond hybrid, Felinem, were studied to characterize their biochemical and molecular responses induced under iron-Deficient conditions. Plants of both genotypes were submitted to different treatments using a hydroponic system that permitted removal of Fe from the nutrient solution. Control plants were grown in 90 ?M Fe (III)-EDTA, Deficient plants were grown in an iron free solution, and plants submitted to an Inductor treatment were resupplied with 180 ?M Fe (III)-EDTA over 1 and 2 days after a period of 4 or 15 days of growth on an iron-free solution. Felinem increased the activity of the iron chelate reductase (FC-R) in the Inductor treatment after 4 days of iron deprivation. In contrast, P 2175 did not show any response after at least 15 days without iron. The induction of the FC-R activity in this genotype was coincident in time with the medium acidification. These results suggest two different mechanisms of iron chlorosis tolerance in both Strategy I genotypes. Felinem would use the iron reduction as the main mechanism to capture the iron from the soil, and in P 2175, the mechanism of response would be slower and start with the acidification of the medium synchronized with the gradual loss of chlorophyll in leaves. To better understand the control of these responses at the molecular level, the differential expression of PFRO2, PIRT1 and PAHA2 genes involved in the reductase activity, the iron transport in roots, and the proton release, respectively, were analyzed. The expression of these genes, estimated by quantitative real-time PCR, was different between genotypes and among treatments. The results were in agreement with the physiological responses observed.

Author(s): 
Gonzalo, María José
Moreno, María Ángeles
Gogorcena, Yolanda
Item Type: 
Journal Article
Publication Title: 
Journal of Plant Physiology
Journal Abbreviation: 
J. Plant Physiol.
Publication Date: 
6/15/2011
Publication Year: 
2011
Pages: 
887-893
Volume: 
168
Issue: 
9
ISSN: 
1618-1328
DOI: 
10.1016/j.jplph.2010.11.017
Library Catalog: 
PubMed
Extra: 
PMID: 21306783

Turabian/Chicago Citation

María José Gonzalo, María Ángeles Moreno and Yolanda Gogorcena. 6/15/2011. "Physiological responses and differential gene expression in Prunus rootstocks under iron deficiency conditions." Journal of Plant Physiology 168: 9: 887-893. 10.1016/j.jplph.2010.11.017.

Wikipedia Citation

<ref> {{Cite journal | doi = 10.1016/j.jplph.2010.11.017 | issn = 1618-1328 | volume = 168 | pages = 887-893 | last = Gonzalo | first = María José | coauthors = Moreno, María Ángeles, Gogorcena, Yolanda | title = Physiological responses and differential gene expression in Prunus rootstocks under iron deficiency conditions | journal = Journal of Plant Physiology | date = 6/15/2011 | pmid = | pmc = }} </ref>