Preventive action of curcumin in experimental acute pancreatitis in mouse

BACKGROUND & OBJECTIVES: Curcuma longa (turmeric) has a long history of use in Ayurvedic medicine as a treatment for inflammatory conditions. The purpose of the present study was to investigate the preventive effects of curcumin against acute pancreatitis (AP) induced by caerulein in mouse and to elucidate possible mechanism of curcumin action. METHODS: Curcumin (50 mg/kg/day) was intraperitoneally injected to Kun Ming male mice for 6 days, followed by injection of caerulein to induce AP. GW9662 (0.3 mg/kg), a specific peroxisome proliferator-activated receptor gamma (PPARγ) antagonist, was intravenously injected along with curcumin. Murine macrophage RAW264.7 cells were treated with 100 μmol/l curcumin for 2 h, and then stimulated with 0.1 μ g/ml lipopolysaccharide (LPS). Serum amylase and transaminase levels were measured at 10 h after AP. TNF-α level in mouse serum and cell culture medium were detected by ELISA. Expression of PPARγ and NF-κB were analyzed by RT-PCR and Western blot. RESULTS: Curcumin significantly decreased the pancreas injury and reversed the elevation of serum amylase, ALT and AST activities and TNF-α level in mice with AP. Curcumin treatment inhibited the elevation of NF-κB-p65 in the nucleus of mouse pancreas AP group and RAW264.7 cells, but significantly increased the expression of PPARγ. GW9662 could abolish the effects of curcumin on serum levels of amylase, ALT, AST, TNF-α, and NF-κB level. INTERPRETATION & CONCLUSIONS: Our results suggest that curcumin could attenuate pancreas tissue and other organ injury by inhibiting the release of inflammatory cytokine TNF-α. These effects may involve upregulation of PPARγ and subsequent downregulation of NF-κB.

Yu, Wen-Guang
Xu, Gang
Ren, Gui-Jie
Xu, Xia
Yuan, Hui-Qing
Qi, Xiao-Li
Tian, Ke-Li
Item Type: 
Journal Article
Publication Title: 
The Indian Journal of Medical Research
Journal Abbreviation: 
Indian J. Med. Res.
Publication Date: 
Nov 2011
Publication Year: 
Library Catalog: 
NCBI Published Medical (?)
PMID: 22199113 PMCID: PMC3249972

Turabian/Chicago Citation

Wen-Guang Yu, Gang Xu, Gui-Jie Ren, Xia Xu, Hui-Qing Yuan, Xiao-Li Qi and Ke-Li Tian. Nov 2011. "Preventive action of curcumin in experimental acute pancreatitis in mouse." The Indian Journal of Medical Research 134: 5: 717-724. 10.4103/0971-5916.91009.

Wikipedia Citation

<ref> {{Cite journal | doi = 10.4103/0971-5916.91009 | issn = 0971-5916 | volume = 134 | pages = 717-724 | last = Yu | first = Wen-Guang | coauthors = Xu, Gang, Ren, Gui-Jie, Xu, Xia, Yuan, Hui-Qing, Qi, Xiao-Li, Tian, Ke-Li | title = Preventive action of curcumin in experimental acute pancreatitis in mouse | journal = The Indian Journal of Medical Research | date = Nov 2011 | pmid = | pmc = }} </ref>