Preventive effects of a traditional Chinese medicine (Sho-saiko-to) on endotoxin-induced cytotoxicity and tumor necrosis factor-alpha production in J774A.1 Cells
Language: 
English
Abstract: 

Sho-saiko-to, one of the most frequently prescribed Kampo medicines, is used clinically to treat chronic hepatitis and has shown confirmed clinical efficacy. The present study investigated whether Sho-saiko-to can suppress cytotoxicity and tumor necrosis factor (TNF)-alpha production in endotoxin-treated J774A.1 cells. Sho-saiko-to (10-20 microg/ml) did not affect the proliferation of J774A.1 cells, while a high concentration (50 microg/ml) of Sho-saiko-to induced a slight reduction in cell viability. Treatment with Sho-saiko-to (10-50 microg/ml) significantly inhibited endotoxin (10 microg/ml)-induced cytotoxicity in J774A.1 cells. In addition, Sho-saiko-to (20 microg/ml) suppressed TNF-alpha production by endotoxin (1 microg/ml)-activated J774A.1 cells. These findings suggest that the Kampo prescription Sho-saiko-to suppresses cytotoxicity or TNF-alpha production in macrophages treated with endotoxin and that it may be useful in improving septic shock symptoms. Sho-saiko-to may therefore protect against some of the various disturbances caused by endotoxins through its ability to inhibit TNF-alpha production in septic shock.

Author(s): 
Sakaguchi, Shuhei
Furusawa, Shinobu
Item Type: 
Journal Article
Publication Title: 
Biological & Pharmaceutical Bulletin
Journal Abbreviation: 
Biol. Pharm. Bull.
Publication Date: 
Sep 2004
Publication Year: 
2004
Pages: 
1468-1470
Volume: 
27
Issue: 
9
ISSN: 
0918-6158
Library Catalog: 
NCBI Published Medical (?)
Extra: 
PMID: 15340242

Turabian/Chicago Citation

Shuhei Sakaguchi and Shinobu Furusawa. Sep 2004. "Preventive effects of a traditional Chinese medicine (Sho-saiko-to) on endotoxin-induced cytotoxicity and tumor necrosis factor-alpha production in J774A.1 Cells." Biological & Pharmaceutical Bulletin 27: 9: 1468-1470.

Wikipedia Citation

<ref> {{Cite journal | doi = | issn = 0918-6158 | volume = 27 | pages = 1468-1470 | last = Sakaguchi | first = Shuhei | coauthors = Furusawa, Shinobu | title = Preventive effects of a traditional Chinese medicine (Sho-saiko-to) on endotoxin-induced cytotoxicity and tumor necrosis factor-alpha production in J774A.1 Cells | journal = Biological & Pharmaceutical Bulletin | date = Sep 2004 | pmid = | pmc = }} </ref>