Proteomic analysis of Terminalia chebula extract-dependent changes in human lymphoblastic T cell protein expression
Language: 
English
Abstract: 

Terminalia chebula is a native plant from southern Asia to southwestern China that is used in traditional medicine for the treatment of malignant tumors and diabetes. This plant also has antibacterial and immunomodulatory properties. The present study assessed T. chebula extract-dependent protein expression changes in Jurkat cells. Matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry and Ingenuity Pathways Analysis (IPA) were performed to assess protein expression and networks, respectively. A comparative proteomic profile was determined in T. chebula extract (50 ?g/mL)-treated and control cells; the expressions of ?-tubulin, ring finger and CHY zinc finger domain containing 1, and insulin-like growth factor 1 receptor kinase were significantly down-regulated in T. chebula extract-treated Jurkat cells. Moreover, the molecular basis for the T. chebula extract-dependent protein expression changes in Jurkat cells was determined by IPA. Treatment with the T. chebula extract significantly inhibited nuclear factor-?B activity and affected the proteomic profile of Jurkat cells. The molecular network signatures and functional proteomics obtained in this study may facilitate the evaluation of potential antitumor therapeutic targets and elucidate the molecular mechanism of T. chebula extract-dependent effects in Jurkat cells.

Author(s): 
Das, Nando Dulal
Jung, Kyoung Hwa
Park, Ji Hyun
Choi, Mi Ran
Lee, Hyung Tae
Kim, Moo Sung
Lee, Sang Rin
Chai, Young Gyu
Item Type: 
Journal Article
Publication Title: 
Journal of Medicinal Food
Journal Abbreviation: 
J Med Food
Publication Date: 
2012-07
Publication Year: 
2012
Pages: 
651-657
Volume: 
15
Issue: 
7
ISSN: 
1557-7600
DOI: 
10.1089/jmf.2011.1998
Library Catalog: 
PubMed
Extra: 
PMID: 22471968 PMCID: PMC3382265

Turabian/Chicago Citation

Nando Dulal Das, Kyoung Hwa Jung, Ji Hyun Park, Mi Ran Choi, Hyung Tae Lee, Moo Sung Kim, Sang Rin Lee and Young Gyu Chai. 2012-07. "Proteomic analysis of Terminalia chebula extract-dependent changes in human lymphoblastic T cell protein expression." Journal of Medicinal Food 15: 7: 651-657. 10.1089/jmf.2011.1998.

Wikipedia Citation

<ref> {{Cite journal | doi = 10.1089/jmf.2011.1998 | issn = 1557-7600 | volume = 15 | pages = 651-657 | last = Das | first = Nando Dulal | coauthors = Jung, Kyoung Hwa, Park, Ji Hyun, Choi, Mi Ran, Lee, Hyung Tae, Kim, Moo Sung, Lee, Sang Rin, Chai, Young Gyu | title = Proteomic analysis of Terminalia chebula extract-dependent changes in human lymphoblastic T cell protein expression | journal = Journal of Medicinal Food | date = 2012-07 | pmid = | pmc = }} </ref>