Regulation of ENaC expression at the cell surface by Rab11
Language: 
English
Abstract: 

The epithelial Na(+) channel (ENaC) is an essential channel responsible for Na(+) reabsorption. Coexpression of Rab11a and Rab3a small G proteins with ENaC results in a significant increase in channel activity. In contrast, coexpression of Rab5, Rab27a, and Arf-1 had no effect or slightly decreased ENaC activity. Inhibition of MEK with PD98059, Rho-kinase with Y27632 or PI3-kinase with LY294002 had no effect on ENaC activity in Rab11a-transfected CHO cells. Fluorescence imaging methods demonstrate that Rab11a colocalized with ENaC. Rab11a increases ENaC activity in an additive manner with dominant-negative dynamin, which is a GTPase responsible for endocytosis. Brefeldin A, an inhibitor of intracellular protein translocation, blocked the stimulatory action of Rab11a on ENaC activity. We conclude that ENaC channels, present on the apical plasma membrane, are being exchanged with channels from the intracellular pool in a Rab11-dependent manner.

Author(s): 
Karpushev, Alexey V.
Levchenko, Vladislav
Pavlov, Tengis S.
Lam, V. Y.
Vinnakota, Kalyan C.
Vandewalle, Alain
Wakatsuki, Tetsuro
Staruschenko, Alexander
Item Type: 
Journal Article
Publication Title: 
Biochemical and Biophysical Research Communications
Journal Abbreviation: 
Biochem. Biophys. Res. Commun.
Publication Date: 
Dec 12, 2008
Publication Year: 
2008
Pages: 
521-525
Volume: 
377
Issue: 
2
ISSN: 
1090-2104
DOI: 
10.1016/j.bbrc.2008.10.014
Library Catalog: 
NCBI Published Medical (?)
Extra: 
PMID: 18926797 PMCID: PMC2612579

Turabian/Chicago Citation

Alexey V. Karpushev, Vladislav Levchenko, Tengis S. Pavlov, V. Y. Lam, Kalyan C. Vinnakota, Alain Vandewalle, Tetsuro Wakatsuki and Alexander Staruschenko. Dec 12, 2008. "Regulation of ENaC expression at the cell surface by Rab11." Biochemical and Biophysical Research Communications 377: 2: 521-525. 10.1016/j.bbrc.2008.10.014.

Wikipedia Citation

<ref> {{Cite journal | doi = 10.1016/j.bbrc.2008.10.014 | issn = 1090-2104 | volume = 377 | pages = 521-525 | last = Karpushev | first = Alexey V. | coauthors = Levchenko, Vladislav, Pavlov, Tengis S., Lam, V. Y., Vinnakota, Kalyan C., Vandewalle, Alain, Wakatsuki, Tetsuro, Staruschenko, Alexander | title = Regulation of ENaC expression at the cell surface by Rab11 | journal = Biochemical and Biophysical Research Communications | date = Dec 12, 2008 | pmid = | pmc = }} </ref>