S1 nuclease sensitivity of a double-stranded telomeric DNA sequence
Language: 
English
Abstract: 

We examined structural properties of poly d(C4A2).d(T2G4), the telomeric DNA sequence of the ciliated protozoan Tetrahymena. Under conditions of high negative supercoiling, poly d(C4A2).d(T2G4) inserted in a circular plasmid vector was preferentially sensitive to digestion with S1 nuclease. Only the C4A2 strand was sensitive to first-strand S1 cutting, with a markedly skewed pattern of hypersensitive sites in tracts of either 46 or 7 tandem repeats. Linear poly d(C4A2).(T2G4) showed no preferential S1 sensitivity, no circular dichroism spectra indicative of a Z-DNA conformation, no unusual Tm, and no unusual migration in polyacrylamide gel electrophoresis. The S1 nuclease sensitivity properties are consistent with a model proposed previously for supercoiled poly d(CT).d(AG) (Pulleyblank et al., Cell 42:271-280, 1985), consisting of a double-stranded, protonated, right-handed underwound helix. We propose that this structure is shared by related telomeric sequences and may play a role in their biological recognition.

Author(s): 
Budarf, M.
Blackburn, E.
Item Type: 
Journal Article
Publication Title: 
Nucleic Acids Research
Journal Abbreviation: 
Nucleic Acids Res.
Publication Date: 
8/11/1987
Publication Year: 
1987
Pages: 
6273-6292
Volume: 
15
Issue: 
15
ISSN: 
0305-1048
Library Catalog: 
NCBI Published Medical (?)
Extra: 
PMID: 2819822 PMCID: PMC306083

Turabian/Chicago Citation

M. Budarf and E. Blackburn. 8/11/1987. "S1 nuclease sensitivity of a double-stranded telomeric DNA sequence." Nucleic Acids Research 15: 15: 6273-6292.

Wikipedia Citation

<ref> {{Cite journal | doi = | issn = 0305-1048 | volume = 15 | pages = 6273-6292 | last = Budarf | first = M. | coauthors = Blackburn, E. | title = S1 nuclease sensitivity of a double-stranded telomeric DNA sequence | journal = Nucleic Acids Research | date = 8/11/1987 | pmid = | pmc = }} </ref>