Screening and selection of high carotenoid producing in vitro tomato cell culture lines for [13C]-carotenoid production
Language: 
English
Abstract: 

Isotopically labeled tomato carotenoids, phytoene, phytofluene, and lycopene, are needed for mammalian bioavailability and metabolism research but are currently commercially unavailable. The goals of this work were to establish and screen multiple in vitro tomato cell lines for carotenoid production, test the best producers with or without the bleaching herbicides, norflurazon and 2-(4-chlorophenyl-thio)triethylamine (CPTA), and to use the greatest carotenoid accumulator for in vitro 13C-labeling. Different Solanum lycopersicum allelic variants for high lycopene and varying herbicide treatments were compared for carotenoid accumulation in callus and suspension culture, and cell suspension cultures of the hp-1 line were chosen for isotopic labeling. When grown with [U]-13C-glucose and treated with CPTA, hp-1 suspensions yielded highly enriched 13C-lycopene with 45% of lycopene in the M+40 form and 88% in the M+35 to M+40 isotopomer range. To the authors' knowledge this is the first report of highly enriched 13C-carotenoid production from in vitro plant cell culture.

Author(s): 
Engelmann, Nancy J.
Campbell, Jessica K.
Rogers, Randy B.
Rupassara, S. Indumathie
Garlick, Peter J.
Lila, Mary Ann
Erdman, John W.
Item Type: 
Journal Article
Publication Title: 
Journal of Agricultural and Food Chemistry
Journal Abbreviation: 
J. Agric. Food Chem.
Publication Date: 
Sep 22, 2010
Publication Year: 
2010
Pages: 
9979-9987
Volume: 
58
Issue: 
18
ISSN: 
1520-5118
DOI: 
10.1021/jf101942x
Library Catalog: 
NCBI Published Medical (?)
Extra: 
PMID: 20731353 PMCID: PMC3172872

Turabian/Chicago Citation

Nancy J. Engelmann, Jessica K. Campbell, Randy B. Rogers, S. Indumathie Rupassara, Peter J. Garlick, Mary Ann Lila and John W. Erdman. Sep 22, 2010. "Screening and selection of high carotenoid producing in vitro tomato cell culture lines for [13C]-carotenoid production." Journal of Agricultural and Food Chemistry 58: 18: 9979-9987. 10.1021/jf101942x.

Wikipedia Citation

<ref> {{Cite journal | doi = 10.1021/jf101942x | issn = 1520-5118 | volume = 58 | pages = 9979-9987 | last = Engelmann | first = Nancy J. | coauthors = Campbell, Jessica K., Rogers, Randy B., Rupassara, S. Indumathie, Garlick, Peter J., Lila, Mary Ann, Erdman, John W. | title = Screening and selection of high carotenoid producing in vitro tomato cell culture lines for [13C]-carotenoid production | journal = Journal of Agricultural and Food Chemistry | date = Sep 22, 2010 | pmid = | pmc = }} </ref>