Screening of Indian aphrodisiac ayurvedic/herbal healthcare products for adulteration with sildenafil, tadalafil and/or vardenafil using LC/PDA and extracted ion LC-MS/TOF
Language: 
English
Abstract: 

Ayurvedic/herbal healthcare products are considered safe under the impression that they are derived from natural products. But recently, there have been several reports worldwide on the adulteration of synthetic PDE-5 inhibitors in aphrodisiac herbal formulations. Therefore, the objective of the present study was to explore the presence of synthetic PDE-5 inhibitors (sildenafil, tadalafil and/or vardenafil) in ayurvedic/herbal healthcare products sold in Indian market for aphrodisiac/related uses. In total, 85 herbal formulations (HFs) were included in the study. The formulations were extracted with methanol and subjected to centrifugation. The supernatant was analysed by HPLC and LC-MS/TOF. Early detection of the presence of sildenafil, tadalafil and vardenafil in the herbal samples was done by the study of extracted ion mass chromatograms at the m/z values of respective parent ions, and two prominent fragments of each. In case of sildenafil and tadalafil, adulteration was also detected by comparing the relative retention times (RR(T)) and UV spectra. Further substantiation was done through comparison of accurate mass spectra with those of the two available standards. Of the 85 HFs tested, only one was eventually found to be adulterated with sildenafil. The extent of adulterant in this sample was determined to the therapeutic dose in the formulation. The study thus indicates emergence of the problem of adulteration of Indian herbal products with PDE-5 inhibitors.

Author(s): 
Savaliya, Akash A.
Shah, Ravi P.
Prasad, Bhagwat
Singh, Saranjit
Item Type: 
Journal Article
Publication Title: 
Journal of Pharmaceutical and Biomedical Analysis
Journal Abbreviation: 
J Pharm Biomed Anal
Publication Date: 
Jul 8, 2010
Publication Year: 
2010
Pages: 
406-409
Volume: 
52
Issue: 
3
ISSN: 
1873-264X
DOI: 
10.1016/j.jpba.2009.05.021
Library Catalog: 
NCBI Published Medical (?)
Extra: 
PMID: 19540696

Turabian/Chicago Citation

Akash A. Savaliya, Ravi P. Shah, Bhagwat Prasad and Saranjit Singh. Jul 8, 2010. "Screening of Indian aphrodisiac ayurvedic/herbal healthcare products for adulteration with sildenafil, tadalafil and/or vardenafil using LC/PDA and extracted ion LC-MS/TOF." Journal of Pharmaceutical and Biomedical Analysis 52: 3: 406-409. 10.1016/j.jpba.2009.05.021.

Wikipedia Citation

<ref> {{Cite journal | doi = 10.1016/j.jpba.2009.05.021 | issn = 1873-264X | volume = 52 | pages = 406-409 | last = Savaliya | first = Akash A. | coauthors = Shah, Ravi P., Prasad, Bhagwat, Singh, Saranjit | title = Screening of Indian aphrodisiac ayurvedic/herbal healthcare products for adulteration with sildenafil, tadalafil and/or vardenafil using LC/PDA and extracted ion LC-MS/TOF | journal = Journal of Pharmaceutical and Biomedical Analysis | date = Jul 8, 2010 | pmid = | pmc = }} </ref>