Screening of medicinal plants for PPAR? and PPAR? activation and evaluation of their effects on glucose uptake and 3T3-L1 adipogenesis
Language: 
English
Abstract: 

Medicinal plants are a rich source of ligands for nuclear receptors. The present study was aimed to screen a collection of plant extracts for PPAR?/?-activating properties and identify the active extract that can stimulate cellular glucose uptake without enhancing the adipogenesis. A reporter gene assay was performed to screen ethanolic extracts of 263 plant species, belonging to 94 families, for activation of PPAR? and PPAR?. Eight extracts showed activation of PPAR?, while 22 extracts showed activation of PPAR?. The extracts of five plants (Daphne gnidium, Illicium anisatum, Juniperus virginiana, Terminalia chebula, and Thymelaea hirsuta) showed activation of both PPAR? and PPAR? and out of them, D. gnidium and T. hirsuta markedly increased PPAR?/? protein expression. All five extracts showed an increase in cellular glucose uptake. Of the five dual agonists, T. chebula and T. hirsuta did not show any increase in differentiation of 3T3-L1 preadipocytes, but I. anisatum caused an increase in adipogenesis, while D. gnidium and J. virginiana were toxic to adipocytes. The adipogenic effect of rosiglitazone was antagonized by T. chebula and T. hirsuta. It was concluded that T. hirsuta and T. chebula retain the property of elevating glucose uptake as PPAR?/? dual agonists without the undesired side effect of adipogenesis. This is the first report to reveal the PPAR?/? dual agonistic action and glucose uptake enhancing property of T. hirsuta and T. chebula.

Author(s): 
Yang, Min Hye
Avula, Bharathi
Smillie, Troy
Khan, Ikhlas A.
Khan, Shabana I.
Item Type: 
Journal Article
Publication Title: 
Planta Medica
Journal Abbreviation: 
Planta Med.
Publication Date: 
2013-08
Publication Year: 
2013
Pages: 
1084-1095
Volume: 
79
Issue: 
12
ISSN: 
1439-0221
DOI: 
10.1055/s-0033-1350620
Library Catalog: 
PubMed
Extra: 
PMID: 23877921

Turabian/Chicago Citation

Min Hye Yang, Bharathi Avula, Troy Smillie, Ikhlas A. Khan and Shabana I. Khan. 2013-08. "Screening of medicinal plants for PPAR? and PPAR? activation and evaluation of their effects on glucose uptake and 3T3-L1 adipogenesis." Planta Medica 79: 12: 1084-1095. 10.1055/s-0033-1350620.

Wikipedia Citation

<ref> {{Cite journal | doi = 10.1055/s-0033-1350620 | issn = 1439-0221 | volume = 79 | pages = 1084-1095 | last = Yang | first = Min Hye | coauthors = Avula, Bharathi, Smillie, Troy, Khan, Ikhlas A., Khan, Shabana I. | title = Screening of medicinal plants for PPAR? and PPAR? activation and evaluation of their effects on glucose uptake and 3T3-L1 adipogenesis | journal = Planta Medica | date = 2013-08 | pmid = | pmc = }} </ref>