Screening for Rho-kinase 2 inhibitory potential of Indian medicinal plants used in management of erectile dysfunction
Language: 
English
Abstract: 

THE AIM OF THE STUDY: Activation of Rho-kinase 2 (ROCK-II) results in contraction of corpus cavernosum smooth muscle and ROCK-II inhibitors relax corpus cavernosum in vitro and in vivo hence, plant extracts capable of inhibiting ROCK-II enzyme may be useful in management of erectile dysfunction (ED). The aim of the study was to screen selected Indian medicinal plants, having similar ethnopharmacological use for ROCK-II inhibition. MATERIALS AND METHODS: Some Indian medicinal plants reported as aphrodisiacs in Ayurveda and modern scientific literature were collected, authenticated and extracted. Direct methanol and successive aqueous extracts of these plants were screened for ROCK-II inhibitory activity using HTRF(®)KinEASE™ STK S2 Kit (Cisbio Bioassays). Relaxant effect of potent extract was recorded on isolated rat corpus cavernosum. RESULTS: Methanolic and successive aqueous extracts of 30 plants were screened for ROCK-II inhibition and 15 herbal extracts showed inhibition ranging between 50 and 88% at 50 μg/mL. While IC(50) of Y-27632, a standard ROCK-II inhibitor, was found to be 163.8 ± 1.2 nM. The Methanolic extract of Terminalia chebula (METC) with IC(50) value of 6.09 ± 0.17 μg/mL was found to be most potent and relaxed isolated rat corpus cavernosum significantly (p<0.01). Chebulagic and chebulinic acid of METC were found to inhibit ROCK-II and might be responsible for the inhibitory potential of the extract. The traditional use of plants like Butea frondosa, Syzygium aromaticum, Butea superba, Chlorophytum borivilianum and Mucuna pruriens, as aphrodisiacs and for male sexual disorder (MSD) might be in part due to the ROCK II inhibitory potential of these plants. CONCLUSION: Some of the Indian medicinal plants have ROCK-II inhibitory potential and those deserve further investigation.

Author(s): 
Goswami, Sumanta Kumar
Pandre, Manoj Kumar
Jamwal, Rohitash
Dethe, Shekhar
Agarwal, Amit
Inamdar, Mohammed Naseeruddin
Item Type: 
Journal Article
Publication Title: 
Journal of Ethnopharmacology
Journal Abbreviation: 
J Ethnopharmacol
Publication Date: 
Dec 18, 2012
Publication Year: 
2012
Pages: 
483-489
Volume: 
144
Issue: 
3
ISSN: 
1872-7573
DOI: 
10.1016/j.jep.2012.07.045
Library Catalog: 
NCBI Published Medical (?)
Extra: 
PMID: 23043981

Turabian/Chicago Citation

Sumanta Kumar Goswami, Manoj Kumar Pandre, Rohitash Jamwal, Shekhar Dethe, Amit Agarwal and Mohammed Naseeruddin Inamdar. Dec 18, 2012. "Screening for Rho-kinase 2 inhibitory potential of Indian medicinal plants used in management of erectile dysfunction." Journal of Ethnopharmacology 144: 3: 483-489. 10.1016/j.jep.2012.07.045.

Wikipedia Citation

<ref> {{Cite journal | doi = 10.1016/j.jep.2012.07.045 | issn = 1872-7573 | volume = 144 | pages = 483-489 | last = Goswami | first = Sumanta Kumar | coauthors = Pandre, Manoj Kumar, Jamwal, Rohitash, Dethe, Shekhar, Agarwal, Amit, Inamdar, Mohammed Naseeruddin | title = Screening for Rho-kinase 2 inhibitory potential of Indian medicinal plants used in management of erectile dysfunction | journal = Journal of Ethnopharmacology | date = Dec 18, 2012 | pmid = | pmc = }} </ref>